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MABS1754 Anti-p38/SAPK2 Antibody, clone 2F11

This Anti-p38 Antibody, clone 2F11 is validated for use in Western Blotting for the detection of SAPK2.

Anti-p38/SAPK2 Antibody, clone 2F11 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: MAP Kinases Gene Symbol: Mapk14, Crk1, Csbp1, Csbp2, Mxi2 UniProt Number: P47811
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MABS1754
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1754
      Replaces05-454
      DescriptionAnti-p38/SAPK2 Antibody, clone 2F11
      Alternate Names
      • Mitogen-activated protein kinase 14
      • CRK1
      • MAP kinase 14
      • MAP kinase p38 alpha
      • MAPK 14
      • MAX-interacting protein 2
      • Mitogen-activated protein kinase p38 alpha
      • p38a
      • p38alpha
      • p38MAPK
      • SAPK2a
      • Stress-activated protein kinase 2a
      Background InformationMitogen-activated protein kinase 14 (EC 2.7.11.24; UniProt P47811; also known as CRK1, MAP kinase 14, MAP kinase p38 alpha, MAPK 14, MAX-interacting protein 2, Mitogen-activated protein kinase p38 alpha, p38a, p38alpha, p38MAPK, SAPK2a, Stress-activated protein kinase 2a) is encoded by the Mapk14 (also known as Crk1, Csbp1, Csbp2, Mxi2) gene (Gene ID 26416) in murine species. p38 mitogen-activated protein kinases (MAPKs) constitute a group of stress-activated serine/threonine-specific kinases (SAPKs), including p38α (MAPK14/SAPK2A), p38β (MAPK11/SAPK2B), p38γ (ERK6/MAPK12/SAPK3), and p38δ (MAPK13/SAPK4), that mediate cellular signal transduction and play a vital role in numerous biological processes. p38α and p38β are ubiquitously expressed, while p38γ and p38δ are differentially expressed depending on tissue type. Sequence comparisons show approximately 60% identity within the p38 group. but only 40-45% to the other three MAP kinase family groups, extracellular signal-regulated kinases (ERKs), c-jun N-terminal or stress-activated protein kinases (JNK/SAPK), and ERK/big MAP kinase 1 (BMK1). All four p38 kinases contain a conserved Thr-Gly-Tyr (TGY) dual phosphorylation motif and are activated by dual kinases called MAP kinase kinases (MKKs).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-p38 Antibody, clone 2F11 is validated for use in Western Blotting for the detection of SAPK2.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected p38/SAPK2 in 10 µg of mouse (C2C12, NIH/3T3, RAW264), rat (L6, PC12), and human (HEK293, HeLa, HepG2, HUVEC, Jurkat) cell lysates, and in 10 µg of mouse brain homogenate.
      Western Blotting Analysis: Representative lots detected p38 in human A549 epidermoid carcinoma cell and WI38-VA13 fibroblast lysates (Song, J.Y., et al. (2010). J. Biol. Chem. 285(12):9067-9076; Song, J.Y., et al. (2009). Cancer Lett. 283(2):135-142).
      Western Blotting Analysis: A representative lot detected p38 in mouse embryonic stem cell lysate (Wu, X., et al. (2007). Dev. Dyn. 236(10):2767-2778).
      Biological Information
      ImmunogenFull-length murine p38/SAPK2a GST fusion protein.
      Clone2F11
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 2F11 recognizes both p38alpha/SAPK2a and p38beta/SAPK2b.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Mapk14
      • Crk1
      • Csbp1
      • Csbp2
      • Mxi2
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~38 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected p38/SAPK2 in 10 µg of A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1754 04055977351538