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MABS1229 Anti-SKP1 Antibody, clone 3F3.1

Anti-SKP1 Antibody, clone 3F3.1 is an antibody against SKP1 for use in Western Blotting, Immunocytochemistry.

Anti-SKP1 Antibody, clone 3F3.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Ubiquitin & Ubiquitin Metabolism Gene Symbol: SKP1, EMC19, OCP2, SKP1A, TCEB1L UniProt Number: P63208
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MABS1229
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABS1229
      DescriptionAnti-SKP1 Antibody, clone 3F3.1
      Alternate Names
      • S-phase kinase-associated protein 1
      • Cyclin-A/CDK2-associated protein p19
      • OCP-2
      • OCP-II
      • Organ of Corti protein 2
      • Organ of Corti protein II, p19A
      • p19skp1
      • RNA polymerase II elongation factor-like protein
      • SIII
      • Transcription elongation factor B polypeptide 1-like
      Background InformationS-phase kinase-associated protein 1 (UniProt P63208; also known as Cyclin-A/CDK2-associated protein p19, OCP-2, OCP-II, Organ of Corti protein 2, Organ of Corti protein II, p19A, p19skp1, RNA polymerase II elongation factor-like protein, SIII, Transcription elongation factor B polypeptide 1-like) is encoded by the SKP1 (also known as EMC19, OCP2, SKP1A, TCEB1L) gene (Gene ID 6500) in human. Polyubiquitination of proteins is mediated by ubiquitin-activating enzymes (E1s), ubiquitin-conjugating enzymes (E2s), and ubiquitin ligases (E3s). There exist RING finger-type, HECT-type, and U-box type of E3 ligases. Skp1 is one of the three core components of the SCF complex (Skp1, Cullin, F-box) and functions as an adapter that bridges the F-box protein and Cullin (CUL1). SCF complex depends on various F-box proteins for specific recognition and recruitment of substrates. SCF ligase plays an important role in regulating the transitions between G1/S and G2/M phases.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-SKP1 Antibody, clone 3F3.1 is an antibody against SKP1 for use in Western Blotting, Immunocytochemistry.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:200 dilution from a representative lot detected SKP1 in HUVECs, A431 and HeLa cells.
      Biological Information
      ImmunogenGST-tagged recombinant human SKP1 internal fragment.
      EpitopeInternal.
      Clone3F3.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityExpected to react with both spliced isoforms of human SKP1 reported by UniProt (P63208).
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SKP1
      • EMC19
      • OCP2
      • SKP1A
      • TCEB1L
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~18 kDa observed. 18.66 kDa (Isoform 1) and 18.06 kDa (Isoform 2) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH/3T3 cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected SKP1 in 10 µg of NIH/3T3 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABS1229 04055977333343