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MABN713 Anti-CA9 Antibody, clone 2D3

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MABN713
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHC, FCMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABN713
      DescriptionAnti-CA9 Antibody, clone 2D3
      Alternate Names
      • Carbonic anhydrase 9
      • Carbonate dehydratase IX
      • Carbonic anhydrase IX
      • CA-IX
      • Membrane antigen MN
      • Renal cell carcinoma-associated antigen G250
      • RCC-associated antigen G250
      • pMW1
      • CA9
      Background InformationCA9 (Carbonic anhydrase 9) is a single-pass type I membrane protein belonging to the alpha-carbonic anhydrase family. CA9 may be involved in the control of cell proliferation and transformation and appears to be a novel specific biomarker that has been shown to have diagnostic utility in identifying cervical dysplasia and carcinoma. CA9 is primarily expressed in carcinoma cell lines and is found on the surface microvilli and in the nucleus.
      References
      Product Information
      FormatAscites
      Control
      • HeLa and A549 cell lysates.
      PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-CA9 Antibody, clone 2D3 is a highly specific mouse monoclonal antibody, that targets Carbonic Anhydrase & has been tested in western blotting, IHC & Flow Cytometry.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Flow Cytometry
      Application NotesImmunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected CA9 in human stomach and colon tissues.

      Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected CA9 in NTERA-2 cells.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified recombinant fragment of human CA9 expressed in E. Coli.
      Clone2D3
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • MN
      • CAIX
      • CA9
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~50 kDa observed. Uncharacterized bands may appear in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa and A549 cell lysates.

      Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected CA9 in HeLa and A549 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN713 04053252918544

      Documentation

      Anti-CA9 Antibody, clone 2D3 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-CA9 Antibody, clone 2D3 Certificates of Analysis

      TitleLot Number
      Anti-CA9, clone 2D3 - QVP1305210 QVP1305210
      Anti-CA9, clone 2D3 - VP1408060 VP1408060
      Anti-CA9, clone 2D3 - VP1901140 VP1901140
      Anti-CA9, clone 2D3 - VP1912270 VP1912270
      Anti-CA9, clone 2D3 - VP2311290 VP2311290
      Anti-CA9, clone 2D3 -VP1511208 VP1511208
      Anti-CA9, clone 2D3 -VP1608179 VP1608179
      Anti-CA9, clone 2D3 Monoclonal Antibody VP1811093
      Anti-CA9, clone 2D3 Monoclonal Antibody VP1707191