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MABN699 Anti-Metadherin Antibody, clone 2F11C3

Detect Metadherin using this mouse monoclonal antibody, Anti-Metadherin Antibody, clone 2F11C3 validated for use in western blotting, IHC & Flow Cytometry.

Anti-Metadherin Antibody, clone 2F11C3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: 3D3, AEG1, LYRIC, MTDH UniProt Number: Q86UE4
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MABN699
100 µL  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHC, FCMAffinity PurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN699
      DescriptionAnti-Metadherin Antibody, clone 2F11C3
      Alternate Names
      • Protein LYRIC
      • 3D3/LYRIC
      • Astrocyte elevated gene-1 protein
      • AEG-1
      • Lysine-rich CEACAM1 co-isolated protein
      • Metadherin
      • Metastasis adhesion protein
      Background InformationMetadherin, or more commonly known as LYRIC, or AEG-1 (Astrocyte elevated gene-1) is a single pass membrane protein that plays a key role in anchorage-independent growth of immortalized melanocytes and astrocytes. Anchorage-independent division is a critical component in tumor cell expansion and metastasis. Similarly, metadherin activates the nuclear factor kappa- B (NF-kappa-B) transcription leading to expression of numerous genes involved in cell growth and expansion, and it is also involved in HIF-1alpha mediated angiogenesis in tumor growth. Finally new research demonstrates that metadherin interacts with RNA-induced silencing complex (RISC) and plays a very important role in RISC and miRNA functions as well. The protein is a key target of research because of its tumor promoting effects in lung, bone and brain cancers. Metadherin is localized to the endoplasmic reticulum and nuclear membranes in normal tissue, thus staining is perinuclear primarily but Metadherin staining can also be seen in the nucleus, at tight junctions and even at the cell surface in certain cancers. Metadherin expression is activated by TNF, and metadherin is expressed in many cells types with the highest levels in muscle-dominating organs such as skeletal muscle, heart, tongue and small intestine and in endocrine glands such as thyroid and adrenal gland. Metadherin is often over expressed in various cancers including breast, brain, prostate, and melanoma and glioblastoma multiforme. EMD-Millipore’s Anti-metadherin mouse monoclonal, clone 2F11C3 has been tested by western blot against several human cancer cell lysates, by flow cytometry against human HeLa cells, and in human paraffin-embedded human liver tissue.
      References
      Product Information
      FormatAffinity Purified
      Control
      • K562 cell lysate
      PresentationPurified mouse monoclonal IgG1 in PBS containing up to 0.1% sodium azide and 50% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationDetect Metadherin using this mouse monoclonal antibody, Anti-Metadherin Antibody, clone 2F11C3 validated for use in western blotting, IHC & Flow Cytometry.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Flow Cytometry
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Metadherin in human breast cancer tissue.

      Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected Metadherin in HeLa cells

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified protein fragment of Human Metadherin expressed in E. coli.
      Clone2F11C3
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • 3D3
      • AEG1
      • LYRIC
      • MTDH
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~70 kDa observed. Uncharacterized bands may appear in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in K562 cell lysate.

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Metadherin in 10 µg of K562 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN699 04053252912382

      Documentation

      Anti-Metadherin Antibody, clone 2F11C3 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Metadherin Antibody, clone 2F11C3 Certificates of Analysis

      TitleLot Number
      Anti-Metadherin, clone 2F11C3 - QVP1304111 QVP1304111
      Anti-Metadherin, clone 2F11C3 - VP1901180 VP1901180
      Anti-Metadherin, clone 2F11C3 -VP1504145 VP1504145
      Anti-Metadherin, clone 2F11C3 -VP1506293 VP1506293
      Anti-Metadherin, clone 2F11C3 -VP1510270 VP1510270
      Anti-Metadherin, clone 2F11C3 -VP1510294 VP1510294
      Anti-Metadherin, clone 2F11C3 -VP1603023 VP1603023
      Anti-Metadherin, clone 2F11C3 -VP1610132 VP1610132