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MABN690 Anti-PPP1CA Antibody, clone 5E9

Anti-PPP1CA Antibody, clone 5E9 is a highly specific mouse monoclonal antibody & that targets Serine/threonine-protein phosphatase & has been tested in western blotting.

Anti-PPP1CA Antibody, clone 5E9 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: PP-1A, PPP1A, PP1alpha UniProt Number: P62136
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MABN690
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABN690
      DescriptionAnti-PPP1CA Antibody, clone 5E9
      Alternate Names
      • Serine/threonine-protein phosphatase PP1-alpha catalytic subunit
      Background InformationSerine/threonine-protein phosphatase PP1-alpha catalytic subunit (PP-1A) encoded by the gene named PPP1CA or PPP1A is one of the three catalytic subunits of protein phosphatase 1 (PP1), one of the major protein phosphatases in the cell. PPP1CA interacts with over 200 regulatory proteins to form highly specific holoenzymes which dephosphorylate a huge variety of target proteins. PPP1CA is critical for cell division, chromatin structure, protein synthesis, glycogen metabolism, muscle and cardiac function as well as the regulation of long-term ionic conductances and even synaptic plasticity. Developmentally it has nearly as many roles with principal ones being as a regulator of neural tube and optic fissure closure as well as enteric neural crest cell (ENCCs) migration during development. PPP1CA can be found both cytoplasmically and nuclear localized depending upon its regulatory partner but PPP1CA is mostly nuclear/nucleoplasm localized. Interestingly in the brain, PPP1CA also functions as a suppressor of learning and memory and as a potential mediator of cognitive decline during aging. EMD-Millipore’s Anti-PPP1CA mouse monoclonal antibody has been tested in western blot on recombinant protein as well as cell lysates from HeLa, HepG2, MCF-7, Jurkat and A549 cells and by direct ELISA against varying concentrations of antigen.
      References
      Product Information
      FormatAscites
      Control
      • HeLa, HepG2, MCF-7, Jurkat, and A549 cell lysates
      PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-PPP1CA Antibody, clone 5E9 is a highly specific mouse monoclonal antibody & that targets Serine/threonine-protein phosphatase & has been tested in western blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenPurified recombinant fragment of human PPP1CA expressed in E. Coli.
      Clone5E9
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • PP-1A
      • PPP1A
      • PP1alpha
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~38 kDa observed. Uncharacterized bands may appear in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa, HepG2, MCF-7, Jurkat, and A549 cell lysates.

      Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected PPP1CA in HeLa, HepG2, MCF-7, Jurkat, and A549 cell lysates.

      Optimal working dilutions must be determined by end user.

      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN690 04053252917424