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MABN670 Anti-LCN1 Antibody, clone 10B10

Anti-LCN1 Antibody, clone 10B10 is a highly specific mouse monoclonal antibody, that targets Lipocalin & has been tested in western blotting, IHC & Immunofluorescence.

Anti-LCN1 Antibody, clone 10B10 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Developmental Neuroscience Gene Symbol: LCN1, VEGP UniProt Number: P31025
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MABN670
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHC, IFMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABN670
      DescriptionAnti-LCN1 Antibody, clone 10B10
      Alternate Names
      • TP
      • PMFA
      • Lipocalin-1
      • Tear lipocalin
      • Tlc
      • Tear prealbumin
      • Von Ebner gland protein
      • VEG protein
      • LCN1
      Background InformationLipocalin-1 or alternatively known as Tear lipocalin (Tlc), Tear prealbumin (TP) or Von Ebner gland protein (VEG) is encoded by the human gene named LCN1/VEGP and expressed in the salivary and lachrymal glands as well as prostate tissue, but recent research has also shown LCN1 to be produced by other glandular tissues such as the pituitary gland. Lipocalin-1 is part of a larger lipocalin protein family of extracellular proteins and shares the ability to bind hydrophobic ligands and lipids but LCN1 also has other abilities such as being an inhibitor of cysteine proteinases and thus plays a role in the control of inflammation. LCN1 also seems to be important in taste utilization and plays a role in the non-immunological defense against micro-organisms and viruses in the eye and mouth. EMD-Millipore’s Anti-LCN1 mouse monoclonal antibody has been tested in western blot against HEK293 transfected LCN1 lysates and in paraffin embedded immunohistochemistry on human prostate tissue sections and in fluorescent immunocytochemistry on transfected HEK293 cells in culture.
      References
      Product Information
      FormatAscites
      Control
      • HEK293 and LCN1-hIgGFc transfected HEK293 cell lysates
      PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-LCN1 Antibody, clone 10B10 is a highly specific mouse monoclonal antibody, that targets Lipocalin & has been tested in western blotting, IHC & Immunofluorescence.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Immunofluorescence
      Application NotesImmunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected LCN1 in methanol-fixed HEK293 cells transfected with LCN1-hIgGFc.

      Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected LCN1 in human prostate tissue.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified recombinant fragment of human LCN1 expressed in E. Coli.
      Clone10B10
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • LCN1
      • VEGP
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~52 kDa observed. Uncharacterized bands may appear in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HEK293 and LCN1-hIgGFc transfected HEK293 cell lysates.

      Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected LCN1-hIgGFc in LCN1-hIgGFc transfected HEK293 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN670 04053252917479