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MABN1615 Anti-Cdh1 Antibody, clone 15A9.1

Anti-Cdh1, clone 15A9.1, Cat. No. MABN1615, is a highly specific mouse monoclonal antibody that targets Cdh1 and has been tested in Western Blotting.

Anti-Cdh1 Antibody, clone 15A9.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: FZR1, CDH1, FYR, FZR, KIAA1242 UniProt Number: Q9UM11
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MABN1615
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWB, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1615
      DescriptionAnti-Cdh1 Antibody, clone 15A9.1
      Alternate Names
      • Fizzy-related protein homolog
      • CDC20-like protein 1
      • CDH1alpha
      • CDH1beta
      • Cdh1/Hct1 homolog
      • hCDH1
      • Fzr
      • Fzr1
      • Fzr2
      Background InformationFizzy-related protein homolog (UniProt Q9UM11; also known as CDC20-like protein 1, Cdh1/Hct1 homolog, hCDH1, Fzr) is encoded by the FZR1 (also known as CDH1, FYR, FZR, KIAA1242) gene (Gene ID 51343) in human. The anaphase-promoting complex/cyclosome (APC/C) is an E3 ubiquitin ligase involved in cell cycle regulation. APC/C is activated upon sequential binding to the Cdc20 and Cdh1 adaptor proteins. Cdc20 associates with APC/C during early mitosis and principally regulates mitotic progression, while Cdh1 interacts with the APC/C from late mitosis onwards until the following G1/S transition. DNA-end resection factor CtIP. Cdk-mediated Cdh1 phosphorylation prevents Cdh1 from associating with APC/C prematurely during S/G2 phase, while Cdc14B phosphatase-dependent APC/C-Cdh1 activation in response to DNA damage during G2 phase leads to Polo-like kinase 1 (Plk1) degradation. Following DNA double-strand breaks (DSB) repair, Cdk1 and Plk1 are reactivated to allow cell cycle progression from G2 into mitosis. Cdh1-depleted cells or Cdh1 / mice exhibit elevated levels of DNA damage and chromosomal aberrations. APC/C-Cdh1 interacts with the KEN box motif on its target proteins and known APC/C-Cdh1 substrates include the the de-SUMOylation enzyme SENP2, and and the fragile X syndrome protein FMRP.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Cdh1, clone 15A9.1, Cat. No. MABN1615, is a highly specific mouse monoclonal antibody that targets Cdh1 and has been tested in Western Blotting.
      Key Applications
      • Western Blotting
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected Cdh1 in 10 µg of embryonic (E13-14 & E16) and neonatal (P1 & P7) mouse brain tissue lysates, as well as in mouse embryonic stem cell (mESC) and neural stem cell (mNSC) lysates.
      Biological Information
      ImmunogenGST-tagged recombinant human Cdh1 N-terminal fragment.
      Clone15A9.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 15A9.1 targeted epitope lies within the N-terminal region of mouse and rat Cdh1, as well as all three human Cdh1 spliced isoforms reported by UniProt (Q9R1K5, B1WCA1, and Q9UM11).
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Predicted to react with Human and Rat based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • FZR1
      • CDH1
      • FYR
      • FZR
      • KIAA1242
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~55 kDa observed. 55.18 kDa (human isoform 1), 54.77 (human isoform 2, CDH1alpha or Fzr1), 44.87 kDa (human isoform 3, CDH1beta or Fzr2), and 54.69 kDa (mouse & rat) calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse E16 brain tissue lysate.

      Western Blotting Analysis: A 1:2,000 dilution of this antibody detected Cdh1 in 10 µg of embryonic E16 mouse brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN1615 04054839057878