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MABN1598 Anti-RetGC-1/GUCY2D Antibody, clone 6D8.2

This Anti-RetGC-1 Antibody, clone 6D8.2 is validated for use in Western Blotting for the detection of GUCY2D.

Anti-RetGC-1/GUCY2D Antibody, clone 6D8.2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Sensory & PNS Gene Symbol: GUCY2D, CORD6, GUC1A4, GUC2D, RETGC, RETGC1 UniProt Number: Q02846
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MABN1598
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1598
      DescriptionAnti-RetGC-1/GUCY2D Antibody, clone 6D8.2
      Alternate Names
      • Retinal guanylyl cyclase 1
      • Guanylate cyclase 2D, retinal
      • RETGC-1
      • Rod outer segment membrane guanylate cyclase
      • ROS-GC
      Background InformationRetinal guanylyl cyclase 1 (EC 4.6.1.2; UniProt Q02846; also known as Guanylate cyclase 2D, retinal, RETGC-1, Rod outer segment membrane guanylate cyclase, ROS-GC) is encoded by the GUCY2D (also known as CORD6, GUC1A4, GUC2D, RETGC, RETGC1) gene (Gene ID 3000) in human. RetGC-1 is a membrane-bound retinal guanylyl cyclase expressed predominantly in the cone and rod photoreceptors in the cone outer segment. RetGC-1 and the related RetGC-2 mediate the synthesis of cyclic 3′, 5′-guanosine monophosphate (cGMP) from guanosine triphosphate in mammalian photoreceptor cells, where RetGC-1 and its associated activator proteins are responsible for the Ca2+-sensitive restoration of cGMP levels after light activation of the phototransduction cascade. RetGC-1 is produced with a signal peptide (a.a. 1-51) sequence, the removal of which yields the mature enzyme with an exracellular domain (a.a. 52-462), followed by a transmembrane segment (a.a. 463-487) and a cytoplasmic tail (a.a. 488-1103) that contains a protein kinase homology domain (a.a. 525-808), a dimerization domain, and the guanylate cyclase catalytic domain (a.a. 880-1010). Heterozygous mutations in the GUCY2D gene have been shown to cause autosomal dominant cone and cone-rod dystrophies (adCODs and adCORDs, respectively), while homozygous or compound heterozygous mutations cause autosomal recessively inherited Leber Congenital Amaurosis (LCA), the most severe form of inherited retinopathy, resulting in total blindness or greatly impaired vision at birth or in early infancy.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-RetGC-1 Antibody, clone 6D8.2 is validated for use in Western Blotting for the detection of GUCY2D.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenGST-tagged recombinant human RetGC-1/GUCY2D kinase domain fragmen.
      EpitopeKinase domain.
      Clone6D8.2
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 6D8.2 targets an epitope in the cytoplasmic kinase domain of human retinal guanylyl cyclase 1 (RetGC-1) encoded by the GUCY2D gene.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • GUCY2D
      • CORD6
      • GUC1A4
      • GUC2D
      • RETGC
      • RETGC1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~120 kDa observed. 114.7/120.1 kDa (mature/pro-form) calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Y79 human retinoblastoma cell lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected RetGC-1/GUCY2D in 10 µg of Y79 human retinoblastoma cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN1598 04055977353525