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MABN1194 Anti-PSD95 Antibody, clone 3H4.3

Anti-PSD95, clone 3H4.3, Cat. No. MABN1194, is a highly specific mouse monoclonal antibody that targets PSD95 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.

Anti-PSD95 Antibody, clone 3H4.3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Dlg4, Dlgh4, Psd95 UniProt Number: P31016
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MABN1194
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, R, HWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1194
      DescriptionAnti-PSD95 Antibody, clone 3H4.3
      Alternate Names
      • Disks large homolog 4
      • PSD95
      • Postsynaptic density protein 95
      • SAP90
      Background InformationDisks large homolog 4 (UniProt: P31016; also known as Postsynaptic density protein 95, PSD-95, Synapse-associated protein 90, SAP-90, SAP90) is encoded by the Dlg4 (also known as Dlgh4, Psd95) gene (Gene ID: 29495) in rat species. PSD95 belong to the family of membrane-associated guanylate kinases (MAGUKs), which also include PSD-93, SAP97 and SAP102. PSD95 is predominantly expressed in the hippocampus CA1 region and prefrontal cortex of the brain, where it localizes in a somatodendritic pattern in the post-synaptic membrane and in presynaptic axon terminals of inhibitory neurons. PDS95 is the major scaffolding protein in the excitatory postsynaptic density (PSD) and a potent regulator of synaptic strength via interactions with a diverse PSD proteins, including NMDA receptors (NMDARs), AMPA receptor (AMPAR) complexes via Stargazin/TARP, adhesion molecules, as well as other scaffolding proteins, such as GKAP and Shank. PSD95 and neuronal nitric-oxide synthase (nNOS) interaction plays a role in maintaining hypersensitivity in acute and chronic pain. The assembly of the GluR6-PSD95-CaMKII complex is reported to mediate injury induced by brain ischemia by facilitating GluR6 serine phosphorylation by CaMKII and the activation of JNK. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons.
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-PSD95, clone 3H4.3, Cat. No. MABN1194, is a highly specific mouse monoclonal antibody that targets PSD95 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected PSD95 in human cerebellum, rat cerebellum, and rat brain stem tissues.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal region of rat PSD95 protein.
      Clone3H4.3
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 3H4.3 detects PSD95 in human and mouse brain.
      IsotypeIgG1κ
      Species Reactivity
      • Mouse
      • Rat
      • Human
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Dlg4
      • Dlgh4
      • Psd95
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~95 kDa observed; 80.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse brain tissue lysate.

      Western Blotting Analysis: 0.5 µg/mL of this antibody detected PSD-95 in 10 µg of mouse brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN1194 04054839153181