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MABN1185 Anti-Tau (4-repeat isoform RD4) Antibody, clone 7D12.1

This Anti-Tau Antibody, clone 7D12.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Tau (4-repeat isoform RD4).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurodegenerative Diseases Gene Symbol: MAPT, MAPTL, MTBT1, TAU UniProt Number: P10636
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MABN1185
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RWB, IH(P)MPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN1185
      DescriptionAnti-Tau (4-repeat isoform RD4) Antibody, clone 7D12.1
      Alternate Names
      • Microtubule-associated protein tau, 4-repeat isoforms
      • Neurofibrillary tangle protein, 4-repeat isoforms
      • Paired helical filament-tau, 4-repeat isoforms
      • PHF-tau
      • PNS-tau
      • Tau-D
      • Tau-E
      • Tau-F
      • Tau-G
      Background InformationMicrotubule-associated protein tau (UniProt P10636; also known as Neurofibrillary tangle protein, Paired helical filament-tau, PHF-tau) is encoded by the MAPT (also known as MAPTL, MTBT1, TAU) gene (Gene ID 4137) in human. Extracellular plaque deposits composed of amyloid-β and intracellular neurofibrillary tangles (NFTs) composed of truncated and hyperphosphorylated tau are two neuropathological hallmarks of Alzheimer's disease (AD). Tau pathology (tauopathy) can cause toxicity when the brain is devoid of amyloid plaques, and tangle pathology correlates better with clinical dementia than amyloid pathology. Abnormal processing of tau by hyperphosphorylation and proteolytic truncation contribute to the toxicity of tau. Tau also undergoes other types of posttranslational modifications, including glycosylation, ubiquitination, glycation, polyamination, nitration, and lysine methylation, which are believed to be important for its non-pathological functions, including polymerization and stabilization of microtubules. Alternative splicings result in multiple Tau isoforms, including five human Tau spliced isoforms with four C-terminal microtubule-binding repeats/domains (RD4) and four spliced isoforms that contain only three microtubule-binding domains (RD3) due to the absence of exon 10-conding sequence. Isoform-specific antibodies are useful tools for analyzing tau isoforms expression and distribution as well as pathological changes in the human brain.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Tau Antibody, clone 7D12.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Tau (4-repeat isoform RD4).
      Key Applications
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected Tau 4-repeat isoforms in Alzheimer's diseased (AD) human brain, as well as human kidney and non-AD brain tissue sections.
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide (VQIINKKLDLSNVQSKC) corresponding to a junction-flanking sequence coded by adjacent exons 9 & 10 of Tau. The immunizing sequence is identical in human, rat, mouse and bovine.
      EpitopeJunction-flanking region coded by adjacent exons 9 & 10.
      Clone7D12.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 7D12.1 targets an epitope sequence present in Tau spliced isoforms with four C-terminal microtubule-binding repeats/domains (RD4), including human isoforms PNS-tau, Tau-D, Tau-E, Tau-F, and Tau-G, but not Tau spliced isoforms that contain only three microtubule-binding domains (RD3) due to the absence of exon 10-conding sequence (human isoforms Fetal-tau, Tau-A, Tau-B, and Tau-C).
      IsotypeIgG1κ
      Species Reactivity
      • Human
      • Rat
      Species Reactivity NoteHuman, Rat. Predicted to react with Bovine, Mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MAPT
      • MAPTL
      • MTBT1
      • TAU
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~45-58 kDa observed. 78,80 kDa (human isoform 1; PNS-tau), 39.88 kDa (human isoform 6; Tau-D), 42.84 kDa (human isoform 7; Tau-E), 45.72 kDa (human isoform 8; Tau-F), 80.81 kDa (human isoform 9; Tau-G) calculated (Met1 removed).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in rat brain tissue cytosol lysate.

      Western Blotting Analysis: 0.1 µg/mL of this antibody detected Tau 4-repeat isoforms in 10 µg of rat brain tissue cytosol lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN1185 04055977351507

      Documentation

      Anti-Tau (4-repeat isoform RD4) Antibody, clone 7D12.1 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Tau (4-repeat isoform RD4) Antibody, clone 7D12.1 Certificates of Analysis

      TitleLot Number
      Anti-Tau (4-repeat isoform RD4), -Q2686872 Q2686872
      Anti-Tau (4-repeat isoform RD4), clone 7D12.1 - 2999495 2999495
      Anti-Tau (4-repeat isoform RD4), clone 7D12.1 - 3238008 3238008
      Anti-Tau (4-repeat isoform RD4), clone 7D12.1 - 3429607 3429607
      Anti-Tau (4-repeat isoform RD4), clone 7D12.1 - 4123318 4123318
      Anti-Tau (4-repeat isoform RD4), clone 7D12.1 Monoclonal Antibody 2930095
      Anti-Tau (4-repeat isoform RD4), clone 7D12.1 Monoclonal Antibody 3067515