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MABF261 Anti-PD-1 Antibody, clone 16A2.1

Anti-PD-1 Antibody, clone 16A2.1 is an antibody against PD-1 for use in Western Blotting, Flow Cytometry.

Anti-PD-1 Antibody, clone 16A2.1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Research Sub-Category: Immunoglobulins & Immunology Gene Symbol: PDCD1, PD1 UniProt Number: Q15116
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MABF261
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, FCMAffinity PurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF261
      DescriptionAnti-PD-1 Antibody, clone 16A2.1
      Alternate Names
      • PD-1
      • Programmed cell death protein 1
      • Protein PD-1
      • hPD-1
      • CD279
      Background InformationProgrammed cell death protein 1 (PD-1) (UniProt Q15116), also known as CD279, hPD-1, hPD-I, hSLE1, Protein PD-1, Systemic lupus erythematosus susceptibility 2) is a type I single transmembrane protein encoded by the PDCD1 gene in human. Maintenance of peripheral tolerance by preventing the development of autoimmunity due to T lymphocytes specific for self-antigens is essential for homeostasis of the immune system. PD-1 receptor CD279 and its ligands PD-L1 (B7-H1, CD274) and PD-L2 (B7-DC; CD273) play a vital role in the induction and maintenance of peripheral tolerance. Upregulated PD-1 expression is seen on conventional CD4+ CD25- T cells (Tconv) when exposed to mesenchymal stem cells, resulting in increased Tconv apoptosis due to PD-1 stimulation by the MSC PD-L2.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified mouse monoclonal IgG2ak in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-PD-1 Antibody, clone 16A2.1 is an antibody against PD-1 for use in Western Blotting, Flow Cytometry.
      Key Applications
      • Western Blotting
      • Flow Cytometry
      Application NotesFlow Cytometry Analysis: 2.0 µg of this antibody from a representative lot detected PD-1 in 1X10E6 Staph enterotoxin B treated human peripheral blood mononuclear cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the extracellular domain of human PD-1.
      EpitopeExtracellular domain
      Clone16A2.1
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • PDCD1
      • PD1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~32 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in TPA/LPS treated THP-1 cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected PD-1 in 10 µg of TPA/LPS treated THP-1 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABF261 04055977168280

      Documentation

      Anti-PD-1 Antibody, clone 16A2.1 Certificates of Analysis

      TitleLot Number
      Anti-PD-1, clone 16A2.1 - 3478265 3478265
      Anti-PD-1, clone 16A2.1 - 4082281 4082281
      Anti-PD-1, clone 16A2.1 -Q2569101 Q2569101
      Anti-PD-1, clone 16A2.1 Monoclonal Antibody 2929105
      Anti-PD-1, clone 16A2.1 Monoclonal Antibody 3009966

      Technical Info

      Title
      Characterization of Estrogen Receptor α Phosphorylation Sites in Breast Cancer Tissue Using the SNAP i.d® 2.0 System
      White Paper: Further considerations of antibody validation and usage.