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MABE1529 Anti-BRD7 Antibody, clone BRM 2D3

This Anti-BRD7 Antibody, clone BRM 2D3 is validated for use in Western Blotting, Immunoprecipitation for the detection of BRD7.

Anti-BRD7 Antibody, clone BRM 2D3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Chromatin Biology Gene Symbol: BRD7, BP75, CELTIX1 UniProt Number: Q9NPI1
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MABE1529
100 μg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IPRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1529
      ReplacesMABE66
      DescriptionAnti-BRD7 Antibody, clone BRM 2D3
      Alternate Names
      • BRD7
      • Bromodomain-containing protein 7
      • 75 kDa bromodomain protein
      • Protein CELTIX-1
      Background InformationBromodomain-containing protein 7 (UniProt Q9NPI1; also known as 75 kDa bromodomain protein, Protein CELTIX-1) is encoded by the BRD7 (also known as BP75, CELTIX1) gene (Gene ID 29117) in human. BRD7 is a ubiquitously expressed single bromodomain-/BRD-containing member of the family of BRD-containing proteins. BRD7 functions as a subunit of the SWI/SNF chromatin-remodeling complex involved in transcription regulation. It also interacts with the well known tumor suppressor p53 to trans-activate genes involved in cell cycle arrest. BRD7 is downregulated in colorectal carcinoma and nasopharyngeal carcinoma, and BRD7 overexpression is reported to inhibit nasopharyngeal carcinoma cell proliferation via cell cycle arrest by transcriptionally regulating ras/MEK/ERK, Rb/E2F, b-catenin and ERK pathways. BRD7 mRNA has been identified as a silencing target of miR-300, overexpression of miR-300 promotes the proliferation, invasion and EMT of osteosarcoma cells through targeting BRD7 mRNA.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-BRD7 Antibody, clone BRM 2D3 is validated for use in Western Blotting, Immunoprecipitation for the detection of BRD7.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesWestern Blotting Analysis: 1.0 µg/mL from a representative lot detected BRD7 in 10 µg of HEK293 and HeLa cell lysates.
      Immunoprecipitation Analysis: A representative lot immunoprecipitated BRD7 from H1299 cell lysate (Courtesy of Thomas Dobner, Institut fur Medizinische Mikrobiologie und Hygiene, Universitat Regensburg, D-93053 Regensburg, Germany).
      Biological Information
      ImmunogenRecombinant human BRD7.
      CloneBRM203
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone BRM 2D3 detects human 75 kDa bromodomain protein, also known as protein CELTIX-1.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • BRD7
      • BP75
      • CELTIX1
      Purification MethodProtein G purified.
      UniProt Number
      Molecular Weight~75 kDa observed. 74.14 kDa (isoform 1), 74.27 kDa (isoform 2) calculated. Added Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected BRD7 in 10 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE1529 04055977355451