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MABE145 Anti-Runx3 Antibody, clone R3-5G4

Use Anti-Runx3 Antibody, clone R3-5G4 (mouse monoclonal antibody) validated in WB, IP to detect Runx3 also known as Runt-related transcription factor 3, Acute myeloid leukemia 2 protein, PEA2-alpha C, PEBP2-alpha C.

Anti-Runx3 Antibody, clone R3-5G4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: RUNX3, AML2, CBFA3, PEBP2A3 UniProt Number: Q13761
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MABE145
100 µL  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE145
      DescriptionAnti-Runx3 Antibody, clone R3-5G4
      Alternate Names
      • Runt-related transcription factor 3
      • Acute myeloid leukemia 2 protein
      • Core-binding factor subunit alpha-3
      • CBF-alpha-3
      • Oncogene AML-2
      • Polyomavirus enhancer-binding protein 2 alpha C subunit
      • PEA2-alpha C
      • PEBP2-alpha C
      • SL3-3 enhancer factor 1 alpha C subunit
      • SL3/AKV core-binding factor alpha C subunit
      Background InformationRunx3 is a member of the runt domain-containing family of transcription factors. A heterodimer of this protein and a beta subunit forms a complex that binds to the core DNA sequence 5'-PYGPYGGT-3' found in a number of enhancers and promoters, and can either activate or suppress transcription. It also interacts with other transcription factors. It functions as a tumor suppressor, and the gene is frequently deleted or transcriptionally silenced in cancer.
      References
      Product Information
      FormatPurified
      Control
      • Daudi cell lysate.
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-Runx3 Antibody, clone R3-5G4 (mouse monoclonal antibody) validated in WB, IP to detect Runx3 also known as Runt-related transcription factor 3, Acute myeloid leukemia 2 protein, PEA2-alpha C, PEBP2-alpha C.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: A representative lot from an independent laboratory detected Runx3 in HCT116, SW620, and SW480 nuclear extracts. (Ito, K., et al. (2008). Cancer Cell. 14(3):226-237.)
      Biological Information
      ImmunogenRecombinant protein corresponding to human Runx3.
      CloneR3-5G4
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RUNX3
      • AML2
      • CBFA3
      • PEBP2A3
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters.

      SUBUNIT STRUCTURE Heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer and through the Runt domain. DNA-binding is increased by heterodimerization. Interacts with TLE1 and SUV39H1. The tyrosine phosphorylated form (via runt domain) interacts with SRC (via protein kinase domain). Interacts with FYN and LCK.

      SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Note: The tyrosine phosphorylated form localizes to the cytoplasm.
      Molecular Weight~44 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in Daudi cell lysate.

      Western Blot Analysis: 0.25 mg/mL of this antibody detected Runx3 in 10 µg Daudi cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABE145 04053252427169

      Documentation

      Anti-Runx3 Antibody, clone R3-5G4 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Runx3 Antibody, clone R3-5G4 Certificates of Analysis

      TitleLot Number
      Anti-Runx3, clone R3-5G4 - 2360879 2360879
      Anti-Runx3, clone R3-5G4 - 2289165 2289165
      Anti-Runx3, clone R3-5G4 - 3240479 3240479
      Anti-Runx3, clone R3-5G4 - NRG1953328 NRG1953328
      Anti-Runx3, clone R3-5G4 -2893216 2893216
      Anti-Runx3, clone R3-5G4 Monoclonal Antibody 3084595
      Anti-Runx3, clone R3-5G4 Monoclonal Antibody 2911996
      Anti-Runx3, clone R3-5G4 Monoclonal Antibody 2935455

      References

      Reference overviewPub Med ID
      RUNX3 attenuates beta-catenin/T cell factors in intestinal tumorigenesis.
      Ito, Kosei, et al.
      Cancer Cell, 14: 226-37 (2008)  2008

      Show Abstract       		18772112 18772112

      Brochure

      Title
      New Products: Volume 3, 2012