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MABD167 Anti-GATA1 Antibody, clone 4F5

Anti-GATA1 Antibody, clone 4F5 is a highly specific mouse monoclonal antibody, that targets GATA & has been tested in western blotting, ICC & IHC.

Anti-GATA1 Antibody, clone 4F5 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Stem Cell Research Research Sub-Category: Pluripotent & Early Differentiation Gene Symbol: GF1, GF-1, NFE1, XLTT, ERYF1, GATA-1, GATA1 UniProt Number: P15976
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MABD167
100 µL  
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      Overview

      Replacement Information

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, IHCMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABD167
      ReplacesMABE470
      DescriptionAnti-GATA1 Antibody, clone 4F5
      Alternate Names
      • Erythroid transcription factor
      • Eryf1
      • GATA-binding factor 1
      • GATA-1
      • GF-1
      • NF-E1 DNA-binding protein
      Background InformationGATA-1 (GATA1), or alternatively Gata-binding factor 1, GF-1, NF-E1 DNA binding protein, and Erythroid transcription factor (EryF1) encoded by the human gene named GATA1/ERYF1/GF1 is transcriptional activator central for erythroid development. GATA1 also plays critical roles in controlling gene expression in other system as well. Research has shown that GATA1 contributes to the level of alpha synuclein gene expression in Parkinson’s disease and over expression of GATA1 in cortical neurons leads to a decrease in the expression of Rab4b and reduces dendrite branching and maturation, and GATA1 expression is elevated in brains with depressive disorder suggesting that GATA1 may be new target for anti-depressant therapies. EMD-Millipore’s Anti-GATA1 mouse monoclonal antibody has been tested in western blot on K562 cell lysates and paraffin embedded immunohistochemistry on pancreatic cancer tissues and in fluorescent immunocytochemistry on K562 cells in culture.
      References
      Product Information
      FormatAscites
      Control
      • K562 cell lysate
      PresentationMouse monoclonal IgG2b ascitic fluid containing up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-GATA1 Antibody, clone 4F5 is a highly specific mouse monoclonal antibody, that targets GATA & has been tested in western blotting, ICC & IHC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Application NotesImmunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected GATA1 in K562 cells.

      Immunohistochemistry Analysis: A 1:200-1,000 dilution from a representative lot detected GATA1 in pancreatic cancer tissue.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenPurified recombinant fragment of human GATA1 expressed in E. Coli.
      Clone4F5
      HostMouse
      IsotypeIgG2b
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • GF1
      • GF-1
      • NFE1
      • XLTT
      • ERYF1
      • GATA-1
      • GATA1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~45 kDa observed. Uncharacterized bands may appear in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in K562 cell lysate.

      Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected GATA1 in K562 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABD167 04053252918100

      Documentation

      Anti-GATA1 Antibody, clone 4F5 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-GATA1 Antibody, clone 4F5 Certificates of Analysis

      TitleLot Number
      Anti-GATA1, clone 4F5 - QVP1305204 QVP1305204
      Anti-GATA1, clone 4F5 - VP2001070 VP2001070
      Anti-GATA1, clone 4F5 -VP1410070 VP1410070
      Anti-GATA1, clone 4F5 -VP1505073 VP1505073
      Anti-GATA1, clone 4F5 -VP1507159 VP1507159
      Anti-GATA1, clone 4F5 -VP1509243 VP1509243
      Anti-GATA1, clone 4F5 -VP1512152 VP1512152
      Anti-GATA1, clone 4F5 -VP1607213 VP1607213
      Anti-GATA1, clone 4F5 Monoclonal Antibody VP1705255