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MAB8129 Anti-Cytomegalovirus Antibody, immediate early, clone 7D12.2

This Anti-Cytomegalovirus Antibody, immediate early, clone 7D12.2 is validated for use in IF, WB, IC, IH for the detection of Cytomegalovirus.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Infectious Diseases Research Sub-Category: Infectious Diseases - Viral
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MAB8129
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIF, WB, ICC, IHCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB8129
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Cytomegalovirus Antibody, immediate early, clone 7D12.2
      Alternate Names
      • CMV
      References
      Product Information
      FormatPurified
      PresentationPurified ascites in 0.02 M Phosphate Buffered Saline, 0.25 M Sodium Chloride, 0.1% Sodium Azide,

      pH 7.6 .
      Quality LevelMQ300
      Applications
      ApplicationThis Anti-Cytomegalovirus Antibody, immediate early, clone 7D12.2 is validated for use in IF, WB, IC, IH for the detection of Cytomegalovirus.
      Key Applications
      • Immunofluorescence
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry
      Applications Not Recommended
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry.

      Immunocytochemistry.

      IFA at 1:3,000-1:6,000 on acetone fixed cells.

      Does not work on paraffin embedded tissue sections.

      Dilute with buffer pH 7.4-7.6 to desired working volume.

      For extensive dilution, protein containing or other stabilizing medium should be used.

      Final working dilutions must be determined by end user.
      Biological Information
      ImmunogenMRC-5 cells infected with CMV AD169.
      Epitopeimmediate early
      Clone7D12.2
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityReacts with an immediate early protein M.W. 68 kD. Can detect CMV infection 1 hour post-infection exhibiting a nuclear and cytoplasmic staining which reaches peak intensity between 8-24 hours.

      With CMV the antigens expressed at different times are listed as:



      Immediate Early (alpha gene expression): those antigens expressed at 3-12 hrs post-infection generally involved in Transcription such as 72kD major phosphoprotein and a few other other antigens at 60 - 80kD.

      Early Antigen (beta genes) a.k.a. Delayed Early or Intermediate Early: expressed at 12-24hrs post-infection. Generally enzymes and one virion structural gene preceding viral DNA synthesis.

      Late Antigen (gamma genes): expressed at 36-48hrs post-infection. Generally structural proteins. Major protein = 55kD
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at +2° to + 8°C for up to 12 months from date of receipt.

      During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB8129 04053252612718

      Documentation

      Anti-Cytomegalovirus Antibody, immediate early, clone 7D12.2 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Cytomegalovirus Antibody, immediate early, clone 7D12.2 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-CMV IMMEDIATE EARLY ANTIGEN - 3679407 3679407
      MOUSE ANTI-CMV IMMEDIATE EARLY ANTIGEN -2528628 2528628
      MOUSE ANTI-CMV IMMEDIATE EARLY ANTIGEN -2538628 2538628
      MOUSE ANTI-CMV IMMEDIATE EARLY ANTIGEN -2571294 2571294
      MOUSE ANTI-CMV IMMEDIATE EARLY ANTIGEN -2573015 2573015
      MOUSE ANTI-CMV IMMEDIATE EARLY ANTIGEN -2574667 2574667
      MOUSE ANTI-CMV IMMEDIATE EARLY ANTIGEN -2576428 2576428
      MOUSE ANTI-CMV IMMEDIATE EARLY ANTIGEN -2836162 2836162