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HSP01 Anti-Hsp72/73 (Ab-1) Mouse mAb (W27)

Anti-Hsp72/73 (Ab-1) Mouse mAb (W27) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Heat Shock Protein 72/73

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HSP01
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, M, RMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~72 kDa constitutive and inducible forms of Hsp72/73 in heat-shocked HeLa cells.
      Catalogue NumberHSP01
      Brand Family Calbiochem®
      SynonymsAnti-Heat Shock Protein 72/73
      References
      ReferencesSmith, M.L., et al. 1993. Carcinogenesis 14, 503.
      Lindquist, S. 1986. Ann. Rev. Biochem. 58, 1151.
      Schlesinger, M.J. 1986. J. Cell Biol. 103, 321.
      Ungewickell, E. 1985. EMBO 4, 3385.
      Welch, W.J. and Feramisco, J.R. 1985. Mol. Cell Biol. 5, 1229.
      Velazquez, J.M. and Lindquist, S. 1984. Cell 36, 655.
      Velazquez, J.M., et al. 1983. J. Cell Biol. 96, 286.
      Schlesinger, M.J., et al. 1982. In Heat Shock: From Bacteria to Man. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 440 pgs.
      Kelley, P. and Schlesinger, M.J. 1978. Cell 15, 1277.
      Lemeaux, P.G., et al. 1978. Cell 13, 427.
      Ritossa, F. 1962. Experientia 18, 571.
      Product Information
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Negative controlUnshocked HeLa cells
      Positive controlHeat-shocked HeLa cells
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Paraffin Sections
      Application NotesImmunoblotting (1-5 µg/ml, chemiluminescence)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      Paraffin Sections (1-10 µg/ml, no pre-treatment required)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpurified Hsp72/73
      CloneW27
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      HSP01 0

      Documentation

      Anti-Hsp72/73 (Ab-1) Mouse mAb (W27) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Hsp72/73 (Ab-1) Mouse mAb (W27) Certificates of Analysis

      TitleLot Number
      HSP01

      References

      Reference overview
      Smith, M.L., et al. 1993. Carcinogenesis 14, 503.
      Lindquist, S. 1986. Ann. Rev. Biochem. 58, 1151.
      Schlesinger, M.J. 1986. J. Cell Biol. 103, 321.
      Ungewickell, E. 1985. EMBO 4, 3385.
      Welch, W.J. and Feramisco, J.R. 1985. Mol. Cell Biol. 5, 1229.
      Velazquez, J.M. and Lindquist, S. 1984. Cell 36, 655.
      Velazquez, J.M., et al. 1983. J. Cell Biol. 96, 286.
      Schlesinger, M.J., et al. 1982. In Heat Shock: From Bacteria to Man. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 440 pgs.
      Kelley, P. and Schlesinger, M.J. 1978. Cell 15, 1277.
      Lemeaux, P.G., et al. 1978. Cell 13, 427.
      Ritossa, F. 1962. Experientia 18, 571.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision27-August-2007 RFH
      SynonymsAnti-Heat Shock Protein 72/73
      ApplicationImmunoblotting (1-5 µg/ml, chemiluminescence)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      Paraffin Sections (1-10 µg/ml, no pre-treatment required)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells. Recognizes the ~72 kDa constitutive and inducible forms of Hsp72/73.
      BackgroundThe heat shock response was first described for Drosophila salivary gland cells, and morphologically consists of a change in their polytene chromosome puffing patterns that involves de novo synthesis of a few proteins. Similar heat shock proteins were later discovered in bacterial, chicken, and mammalian cells, and were subsequently studied in most organisms. A series of heatshock proteins Hsp 90, Hsp 70, Hsp 20-30 and ubiquitin (8,000 m.w.) are induced by insults such as temperature shock, chemicals and other environmental stress. Hsp 70 levels increase 1000-fold in mammalian and Drosophila cells following induction. This protein concentrates in nuclei during heat shock and returns to the cytoplasm when the shock is removed. The exact function(s) of the Hsp 70 and other heat shock proteins are not known. Mammalian Hsp 72/73 is reported to be structurally and functionally related to a protein that releases clathrin triskelia from coated vesicles; Hsp 72/73 is known to bind ATP without hydrolyzing it.
      HostMouse
      Immunogenpurified Hsp72/73
      CloneW27
      IsotypeIgG2a
      Specieshuman, mouse, rat
      Positive controlHeat-shocked HeLa cells
      Negative controlUnshocked HeLa cells
      FormLiquid
      FormulationIn 0.05 M sodium phosphate buffer, 0.2% gelatin.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesSmith, M.L., et al. 1993. Carcinogenesis 14, 503.
      Lindquist, S. 1986. Ann. Rev. Biochem. 58, 1151.
      Schlesinger, M.J. 1986. J. Cell Biol. 103, 321.
      Ungewickell, E. 1985. EMBO 4, 3385.
      Welch, W.J. and Feramisco, J.R. 1985. Mol. Cell Biol. 5, 1229.
      Velazquez, J.M. and Lindquist, S. 1984. Cell 36, 655.
      Velazquez, J.M., et al. 1983. J. Cell Biol. 96, 286.
      Schlesinger, M.J., et al. 1982. In Heat Shock: From Bacteria to Man. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY. 440 pgs.
      Kelley, P. and Schlesinger, M.J. 1978. Cell 15, 1277.
      Lemeaux, P.G., et al. 1978. Cell 13, 427.
      Ritossa, F. 1962. Experientia 18, 571.