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GR09L Anti-Transferrin Receptor (Ab-2) Mouse mAb (42/6)

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-TR, Anti-CD71

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GR09L
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      H, Rb, RMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognizes the ~90 kDa (monomeric) and the ~180 kDa (dimeric) transferrin receptor in the plasma membrane in proliferating cells.
      Catalogue NumberGR09L
      Brand Family Calbiochem®
      SynonymsAnti-TR, Anti-CD71
      References
      ReferencesSchneider, C., et al. 1982. J. Biol. Chem. 257, 8516.
      Trowbridge, I.S. and Omary, M.B. 1981. Proc. Natl. Acad. Sci. USA 78, 3039.
      Aisen, P. and Listowsky, I. 1980. Ann. Rev. Biochem. 49, 357.
      Omary, M.B., et al. 1980. Nature 286, 888.
      Seligman, P.A., et al. 1979. J. Biol. Chem. 254, 9943.
      Product Information
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      Positive controlMost cell lines or placental tissue
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesOriginal Clone, Neutralization Studies Trowbridge, I.S. and Lopez, F. 1982. Proc. Natl. Acad. Sci. USA 79, 1175. Stimulation of Transferin and Iron Uptake McArdle, H.J. and Morgan, E.H. 1984. J. Biol. Chem. 259, 1629.
      Key Applications Immunofluorescence
      Immunoprecipitation
      Neutralization Studies
      Application NotesImmunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      Neutralization Studies (1-2 µg/ml, see comments and application references)
      Application CommentsThis monoclonal antibody has been reported to block binding of transferrin to its receptor and inhibit the growth of a human T-cell line (see application references) as well as to stimulate transferrin and iron uptake in rat and rabbit reticulocytes by increasing the rate of receptor turnover (see application references). This antibody will not bind directly to protein A or protein G PLUS. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpurified, human transferrin receptor
      ImmunogenHuman
      Clone42/6
      HostMouse
      IsotypeIgA
      Species Reactivity
      • Human
      • Rabbit
      • Rat
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsResuspend the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add sodium azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Freezing of aliquots is best for long-term storage of reconstituted product; avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      GR09L 0

      Documentation

      Anti-Transferrin Receptor (Ab-2) Mouse mAb (42/6) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Transferrin Receptor (Ab-2) Mouse mAb (42/6) Certificates of Analysis

      TitleLot Number
      GR09L

      References

      Reference overview
      Schneider, C., et al. 1982. J. Biol. Chem. 257, 8516.
      Trowbridge, I.S. and Omary, M.B. 1981. Proc. Natl. Acad. Sci. USA 78, 3039.
      Aisen, P. and Listowsky, I. 1980. Ann. Rev. Biochem. 49, 357.
      Omary, M.B., et al. 1980. Nature 286, 888.
      Seligman, P.A., et al. 1979. J. Biol. Chem. 254, 9943.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision24-August-2007 RFH
      SynonymsAnti-TR, Anti-CD71
      ApplicationImmunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/sample)
      Neutralization Studies (1-2 µg/ml, see comments and application references)
      DescriptionPurified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with S194/5XXO.BU.1 mouse myeloma cells (see application references). Recognizes the ~90 kDa (monomeric) and the ~180 kDa (dimeric) transferrin receptor in the plasma membrane in proliferating cells.
      BackgroundTransferrin is the major iron binding protein in human plasma and is bound by a specific cell surface receptor during transport of iron into various tissues. The human transferrin receptor, also called CD71, was originally isolated from placenta and shown to contain a subunit with an apparent molecular weight of 90,000. Further structural characterization of the receptor revealed that it resides in the plasma membrane as a 180,000 dimer, has a pI of 5.2, is phosphorylated predominantly on serine residues and contains both complex and high mannose oligosaccharide chains. Transferrin receptor is considered to be a marker for proliferating cells and its levels can be drastically reduced by agents which cause differentiation of undifferentiated cells such as HL-60 cells. Since many of the enzymes of metabolically active, proliferating cells require iron to catalyze redox reactions it is not surprising that the transferrin receptor would be a marker of proliferation. Further support for this hypothesis has been obtained by using antibodies to block transferrin binding and cause the accumulation of cells in S-phase, presumably due to interference with normal iron uptake and energy metabolism.
      HostMouse
      Immunogen speciesHuman
      Immunogenpurified, human transferrin receptor
      Clone42/6
      IsotypeIgA
      Specieshuman, rabbit, rat
      Positive controlMost cell lines or placental tissue
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      PreservativeNone
      CommentsThis monoclonal antibody has been reported to block binding of transferrin to its receptor and inhibit the growth of a human T-cell line (see application references) as well as to stimulate transferrin and iron uptake in rat and rabbit reticulocytes by increasing the rate of receptor turnover (see application references). This antibody will not bind directly to protein A or protein G PLUS. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsResuspend the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add sodium azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Freezing of aliquots is best for long-term storage of reconstituted product; avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesSchneider, C., et al. 1982. J. Biol. Chem. 257, 8516.
      Trowbridge, I.S. and Omary, M.B. 1981. Proc. Natl. Acad. Sci. USA 78, 3039.
      Aisen, P. and Listowsky, I. 1980. Ann. Rev. Biochem. 49, 357.
      Omary, M.B., et al. 1980. Nature 286, 888.
      Seligman, P.A., et al. 1979. J. Biol. Chem. 254, 9943.
      Application referencesOriginal Clone, Neutralization Studies Trowbridge, I.S. and Lopez, F. 1982. Proc. Natl. Acad. Sci. USA 79, 1175. Stimulation of Transferin and Iron Uptake McArdle, H.J. and Morgan, E.H. 1984. J. Biol. Chem. 259, 1629.