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DR1106 Anti-MSH6 Mouse mAb (1F2)

This Anti-MSH6 Mouse mAb (1F2) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of MSH6.

Anti-MSH6 Mouse mAb (1F2) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-MutS Homolog 6

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DR1106
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      OverviewRecognizes the ~140-150 kDa MSH6 protein in HeLa cells and human kidney tissue.
      Catalogue NumberDR1106
      Brand Family Calbiochem®
      SynonymsAnti-MutS Homolog 6
      Application Data
      Detection of human MSH6 by immunoblotting. Sample: Whole tissue extract from human kidney. Primary antibody: Anti-MSH6 Mouse mAb (1F2) (Cat. No. DR1106) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human MSH6 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-MSH6 Mouse mAb (1F2) (Cat. No. DR1106) (10 µg/ml). Detection: fluorescence.
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlHuman kidney tissue, HeLa cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenA recombinant polypeptide corrresponding to amino acids of 931-1031 human MSH6, expressed as a GST fusion protein
      Clone1F2
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DR1106 0

      Documentation

      Anti-MSH6 Mouse mAb (1F2) SDS

      Title

      Safety Data Sheet (SDS) 

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision05-November-2010 RFH
      SynonymsAnti-MutS Homolog 6
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (10 µg/ml)
      Application Data
      Detection of human MSH6 by immunoblotting. Sample: Whole tissue extract from human kidney. Primary antibody: Anti-MSH6 Mouse mAb (1F2) (Cat. No. DR1106) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human MSH6 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-MSH6 Mouse mAb (1F2) (Cat. No. DR1106) (10 µg/ml). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~140-150 kDa MSH6 protein.
      BackgroundMSH6 combines with MSH2 to form a mismatch recognition complex that functions as a bidirectional molecular switch that exchanges ADP and ATP as DNA mismatches are bound and dissociated. Mutations in this gene have been identified in individuals with hereditary nonpolyposis colon cancer (HNPCC) and endometrial cancer.
      HostMouse
      ImmunogenA recombinant polypeptide corrresponding to amino acids of 931-1031 human MSH6, expressed as a GST fusion protein
      Clone1F2
      IsotypeIgG₁
      Specieshuman
      Positive controlHuman kidney tissue, HeLa cells
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review