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DP15L Anti-EBNA Mouse mAb (E8.26)

Anti-EBNA, mouse monoclonal, clone E8.26, recognizes the ~88 kDa wild-type EBNA from EBV-transformed human cells and the ~31.5 kDa mutant EBNA in Raji cells. It is validated for use in WB, IF & IP.

Anti-EBNA Mouse mAb (E8.26) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Epstein Barr Nuclear Antigen

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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      MaMMonoclonal Antibody
      Description
      OverviewRecognizes the ~88 kDa wild-type EBNA from EBV-transformed human cells as well as the ~31.5 kDa mutant form of EBNA in Raji cells.
      Catalogue NumberDP15L
      Brand Family Calbiochem®
      SynonymsAnti-Epstein Barr Nuclear Antigen
      References
      ReferencesFreese, U.K., et al. 1983. J. Virol. 48, 731.
      Fischer, D.K., et al. 1981. Cell 24, 543.
      Orellana, T., et al. 1977. J. Virol. 22, 321.
      Lindahl, T., et al. 1976 J. Mol. Biol. 102, 511.
      Henle, W., et al. 1967 Science 157, 1064.
      Product Information
      FormLyophilized
      FormulationLyophilized from 20 mM ammoinum bicarbonate, 100µg BSA.
      Negative controlHL60 cells
      Positive controlRaji cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesOriginal Clone Hearing, J.C., et al. 1985. Virol. 142, 215.
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Application NotesImmunoblotting (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-5 µg/sample)
      Application CommentsAntibody should be titrated for optimal results in individual systems.
      Biological Information
      ImmunogenEBNA protein
      CloneE8.26
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Mammals
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DP15L 0

      Documentation

      Anti-EBNA Mouse mAb (E8.26) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-EBNA Mouse mAb (E8.26) Certificates of Analysis

      TitleLot Number
      DP15L

      References

      Reference overview
      Freese, U.K., et al. 1983. J. Virol. 48, 731.
      Fischer, D.K., et al. 1981. Cell 24, 543.
      Orellana, T., et al. 1977. J. Virol. 22, 321.
      Lindahl, T., et al. 1976 J. Mol. Biol. 102, 511.
      Henle, W., et al. 1967 Science 157, 1064.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-August-2007 RFH
      SynonymsAnti-Epstein Barr Nuclear Antigen
      ApplicationImmunoblotting (1-5 µg/ml)
      Immunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-5 µg/sample)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3 x 63Ag 8.653 mouse myeloma cells (see application references). Recognizes the ~88 kDa wild-type and the ~31.5 kDa mutant EBNA proteins.
      BackgroundEBNA, Epstein Barr Virus Nuclear Antigen, is viral encoded and present in all EBV-infected cells. The genomes of different EBV isolates are distinguished by characteristic deletions and restriction polymorphisms. The function of this protein is still unknown. Some potential functions include a role in the replication of the episomal viral DNA, regulation of viral gene expression, and involvement in the growth transformation of B lymphocytes.
      HostMouse
      ImmunogenEBNA protein
      CloneE8.26
      IsotypeIgG₁
      SpeciesEBV-infected mammalian B lymphocytes
      Positive controlRaji cells
      Negative controlHL60 cells
      FormLyophilized
      FormulationLyophilized from 20 mM ammoinum bicarbonate, 100µg BSA.
      PreservativeNone
      CommentsAntibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesFreese, U.K., et al. 1983. J. Virol. 48, 731.
      Fischer, D.K., et al. 1981. Cell 24, 543.
      Orellana, T., et al. 1977. J. Virol. 22, 321.
      Lindahl, T., et al. 1976 J. Mol. Biol. 102, 511.
      Henle, W., et al. 1967 Science 157, 1064.
      Application referencesOriginal Clone Hearing, J.C., et al. 1985. Virol. 142, 215.