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DP14L Anti-SV40 T Antigen (Ab-3) Mouse mAb (PAb280)

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
DP14L
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      Sv40 Infected CellsMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.




      Recognizes the ~21 kDa SV40 small T-antigen in SV80 cells. Does not cross-react with SV40 large T-antigen.
      Catalogue NumberDP14L
      Brand Family Calbiochem®
      References
      ReferencesMontano, X. and Lane, D.P. 1989. J. Virol. 63, 3128.
      DeCaprio, J.A., et al. 1988. Cell 54, 275.
      Whyte, P., et al. 1988. Nature 334, 124.
      Mitchell, P.J., et al. 1987. Cell 50, 847.
      Montano, X. and Lane, D.P. 1984. J. Virol. 51, 760.
      Sarnow, P., Ho, et al. 1982. Cell 28, 387.
      Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 41.
      Lane, D.P. and Crawford, L.V. 1979. Nature 278, 261.
      Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA 71, 3754.
      Tooze, J. 1973. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
      Tegtmeyer, P. 1972. J. Virol. 10, 591.
      Product Information
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      Negative controlA431 or NIH3T3 cells
      Positive controlSV80 cells
      PreservativeNone
      Applications
      Application ReferencesOriginal Clone Montano, X. and Lane, D.P. 1984. J. Virol. 51, 760. Immunocytochemistry Tiemann, F., et al. 1995. J. Virol. 69, 6115. Montano, X. and Lane, D.P. 1989. J. Virol. 63, 3128. Montano, X. and Lane, D.P. 1984. J. Virol. 51, 760.
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Immunoprecipitation
      Paraffin Sections
      Application NotesFrozen Sections (1-5 µg/ml)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (see application references)
      Immunoprecipitation (1-2 µg/sample)
      Paraffin Sections (1-10 µg/ml)
      Application CommentsAnti-SV40 T Ag (Ab-3) is reactive by immunoprecipitation and immunoblotting with the 20,500 dalton SV40 small T antigen and stains SV40 infected cells. Does not cross-react with SV40. Antibody should be titrated for optimal results in individual sample types.
      Biological Information
      Immunogenthe SV40 small T-antigen
      ClonePAb280
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Sv40 Infected Cells
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline, pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DP14L 0

      Documentation

      Anti-SV40 T Antigen (Ab-3) Mouse mAb (PAb280) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-SV40 T Antigen (Ab-3) Mouse mAb (PAb280) Certificates of Analysis

      TitleLot Number
      DP14L

      References

      Reference overview
      Montano, X. and Lane, D.P. 1989. J. Virol. 63, 3128.
      DeCaprio, J.A., et al. 1988. Cell 54, 275.
      Whyte, P., et al. 1988. Nature 334, 124.
      Mitchell, P.J., et al. 1987. Cell 50, 847.
      Montano, X. and Lane, D.P. 1984. J. Virol. 51, 760.
      Sarnow, P., Ho, et al. 1982. Cell 28, 387.
      Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 41.
      Lane, D.P. and Crawford, L.V. 1979. Nature 278, 261.
      Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA 71, 3754.
      Tooze, J. 1973. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
      Tegtmeyer, P. 1972. J. Virol. 10, 591.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-March-2011 JSW
      ApplicationFrozen Sections (1-5 µg/ml)
      Immunoblotting (1-5 µg/ml)
      Immunocytochemistry (see application references)
      Immunoprecipitation (1-2 µg/sample)
      Paraffin Sections (1-10 µg/ml)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with SP20/AG14 mouse myeloma cells (see application references). Recognizes the ~25 kDa SV40 T antigen protein.
      BackgroundBoth the SV40 large T and small T antigens are encoded by the early region of the SV40 genome. The large T antigen binds DNA, and complexes with a 53,000 dalton cellular transforming protein, p53, which is required for initiation of viral DNA replication during lytic growth. In addition, the large T antigen binds DNA polymerase and the transcription factor AP-2 and forms a specific complex with the p105 product of the retinoblastoma susceptibility gene.
      HostMouse
      Immunogenthe SV40 small T-antigen
      ClonePAb280
      IsotypeIgG₁
      SpeciesSV40 infected cells
      Positive controlSV80 cells
      Negative controlA431 or NIH3T3 cells
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      PreservativeNone
      CommentsAnti-SV40 T Ag (Ab-3) is reactive by immunoprecipitation and immunoblotting with the 20,500 dalton SV40 small T antigen and stains SV40 infected cells. Does not cross-react with SV40. Antibody should be titrated for optimal results in individual sample types.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline, pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesMontano, X. and Lane, D.P. 1989. J. Virol. 63, 3128.
      DeCaprio, J.A., et al. 1988. Cell 54, 275.
      Whyte, P., et al. 1988. Nature 334, 124.
      Mitchell, P.J., et al. 1987. Cell 50, 847.
      Montano, X. and Lane, D.P. 1984. J. Virol. 51, 760.
      Sarnow, P., Ho, et al. 1982. Cell 28, 387.
      Crawford, L.V., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 41.
      Lane, D.P. and Crawford, L.V. 1979. Nature 278, 261.
      Carroll, R.B., et al. 1974. Proc. Natl. Acad. Sci. USA 71, 3754.
      Tooze, J. 1973. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
      Tegtmeyer, P. 1972. J. Virol. 10, 591.
      Application referencesOriginal Clone Montano, X. and Lane, D.P. 1984. J. Virol. 51, 760. Immunocytochemistry Tiemann, F., et al. 1995. J. Virol. 69, 6115. Montano, X. and Lane, D.P. 1989. J. Virol. 63, 3128. Montano, X. and Lane, D.P. 1984. J. Virol. 51, 760.