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DP10L Anti-Polyoma Virus T Antigen (Ab-4) Mouse mAb (F4)

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DP10L
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      Polyoma Infected CellsMMonoclonal Antibody
      Description
      Overview

      This product has been discontinued.



      Recognize polyoma virus large, middle, and small T antigens.
      Catalogue NumberDP10L
      Brand Family Calbiochem®
      References
      ReferencesBolen, J.B., et al. 1984. Cell 38, 767.
      Courtneidge, S.A. and Smith A.E. 1984. EMBO J. 3, 585.
      Courtneidge, S.A. and Smith, A.E. 1983. Nature 303, 435.
      Eckhart, W. 1981. Adv. Cancer Res. 35, 1.
      Ito, Y., et al. 1980. J. Virol. 35, 219.
      Ito, Y. and Spurr, N. 1980. Cold Spring Harbor Symp. Quant. Biol. 44, 149.
      Turler, H. 1980. Mol. Cell. Biochem. 32, 63.
      Smith, A.E., et al. 1979. Cell 18, 915.
      Eckhart, W., et al. 1979. Cell 18, 925.
      Schaffhausen, B.S. and Benjamin, T.L. 1979. Cell 18, 935.
      Product Information
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      Positive controlPolyoma infected 3T6 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesOriginal Clone Pallas, D., et al. 1986. J. Virol. 60, 1075.
      Key Applications Immunofluorescence
      Immunoprecipitation
      Not Western Blot
      Application NotesImmunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/ml)
      Immunoblotting (not recommended)
      Application CommentsFor immunoprecipitation, label by autophosphorylation. Antibody should be titrated for optimal results in individual sample types.
      Biological Information
      ImmunogenPolyoma virus T antigen
      CloneF4
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Polyoma Infected Cells
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsResuspend the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline, pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      DP10L 0

      Documentation

      Anti-Polyoma Virus T Antigen (Ab-4) Mouse mAb (F4) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Polyoma Virus T Antigen (Ab-4) Mouse mAb (F4) Certificates of Analysis

      TitleLot Number
      DP10L

      References

      Reference overview
      Bolen, J.B., et al. 1984. Cell 38, 767.
      Courtneidge, S.A. and Smith A.E. 1984. EMBO J. 3, 585.
      Courtneidge, S.A. and Smith, A.E. 1983. Nature 303, 435.
      Eckhart, W. 1981. Adv. Cancer Res. 35, 1.
      Ito, Y., et al. 1980. J. Virol. 35, 219.
      Ito, Y. and Spurr, N. 1980. Cold Spring Harbor Symp. Quant. Biol. 44, 149.
      Turler, H. 1980. Mol. Cell. Biochem. 32, 63.
      Smith, A.E., et al. 1979. Cell 18, 915.
      Eckhart, W., et al. 1979. Cell 18, 925.
      Schaffhausen, B.S. and Benjamin, T.L. 1979. Cell 18, 935.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-August-2007 RFH
      ApplicationImmunofluorescence (1-5 µg/ml)
      Immunoprecipitation (1-2 µg/ml)
      Immunoblotting (not recommended)
      DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~100 kDa large T antigen, the ~55 kDa middle T antigen, and the ~22 kDa small T antigen of polyoma virus.
      BackgroundPolyoma virus T-Ag (Ab-4) is a broadly reactive monoclonal antibody which recognizes the large, middle and small T antigens in polyoma virus transformed or infected cells. Polyoma virus is a small DNA tumor virus capable of lytically infecting mouse cells in culture and of transforming other rodent cells (rat and hamster fibroblasts, predominantly). The "early region" of polyoma virus DNA codes for three major polypeptides. These proteins have been designated large T antigen (~100 kDa), middle T antigen (~55 kDa) and small T antigen (~22 kDa). These large, middle, and small T antigens share N-terminal amino acid sequences. However, because the middle T antigen mRNA is spliced into a different reading frame from the large T antigen at the C-terminal portion of the two proteins, they exhibit distinct differences in cellular location and function.
      HostMouse
      ImmunogenPolyoma virus T antigen
      CloneF4
      IsotypeIgG₁
      Speciespolyoma infected cells
      Positive controlPolyoma infected 3T6 cells
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      PreservativeNone
      CommentsFor immunoprecipitation, label by autophosphorylation. Antibody should be titrated for optimal results in individual sample types.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsResuspend the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline, pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesBolen, J.B., et al. 1984. Cell 38, 767.
      Courtneidge, S.A. and Smith A.E. 1984. EMBO J. 3, 585.
      Courtneidge, S.A. and Smith, A.E. 1983. Nature 303, 435.
      Eckhart, W. 1981. Adv. Cancer Res. 35, 1.
      Ito, Y., et al. 1980. J. Virol. 35, 219.
      Ito, Y. and Spurr, N. 1980. Cold Spring Harbor Symp. Quant. Biol. 44, 149.
      Turler, H. 1980. Mol. Cell. Biochem. 32, 63.
      Smith, A.E., et al. 1979. Cell 18, 915.
      Eckhart, W., et al. 1979. Cell 18, 925.
      Schaffhausen, B.S. and Benjamin, T.L. 1979. Cell 18, 935.
      Application referencesOriginal Clone Pallas, D., et al. 1986. J. Virol. 60, 1075.