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CP70 Anti-Fibronectin (Ab-3) Mouse mAb (FBN11)

Anti-Fibronectin (Ab-3), mouse monoclonal, clone FBN11, recognizes dimeric fibronectin in SWI56 cells and normal kidney. It is validated for use in WB, IF, IP & IHC (frozen and paraffin sections).

Anti-Fibronectin (Ab-3) Mouse mAb (FBN11) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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CP70
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, M, RMMonoclonal Antibody

Pricing & Availability

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CP70-100UG
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      		 Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~440 kDa disulfide-linked, dimeric fibronectin in SWI56 cells and normal kidney tissue.
      Catalogue NumberCP70
      Brand Family Calbiochem®
      References
      ReferencesClark, R.A. 2001. Ann. N.Y. Acad. Sci. 936, 355.
      Armstrong, P.B. and Armstrong, M.T. 2000. Biochim. Biophys. Acta 1470, O9.
      Barilla, M.L. and Carsons, S.E. 2000. Semin. Arthritis Rheum. 29, 252.
      Yamada, K.M. 1989. In Fibronectin, Mosher, D.F., ed., p. 47.
      Rouslahti, E. and Pierschbacher, M.D. 1986. Cell 44, 517.
      Pytela, R., et al. 1985. Cell 40, 191.
      Product Information
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
      Negative controlNormal mouse serum
      Positive controlSW 156 cells or normal kidney tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Frozen Sections
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Paraffin Sections
      Application NotesFrozen Sections (0.5-1 µg/ml)
      Immunoblotting (0.5-1 µg/ml)
      Immunofluorescence (see comments)
      Immunoprecipitation (2 µg/mg protein lysate)
      Paraffin Sections (0.5-1 µg/ml, heat pre-treatment required)
      Application CommentsExhibits staining of the connective tissue matrix when used in immunohistochemistry. This antibody is suitable for both reduced and non-reduced protein by immunoblotting. For staining of formalin-fixed tissues, boil in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. This antibody has also been reported to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant human fibronectin
      ImmunogenHuman
      CloneFBN11
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CP70-100UG 04055977221015

      Documentation

      Anti-Fibronectin (Ab-3) Mouse mAb (FBN11) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Fibronectin (Ab-3) Mouse mAb (FBN11) Certificates of Analysis

      TitleLot Number
      CP70

      References

      Reference overview
      Clark, R.A. 2001. Ann. N.Y. Acad. Sci. 936, 355.
      Armstrong, P.B. and Armstrong, M.T. 2000. Biochim. Biophys. Acta 1470, O9.
      Barilla, M.L. and Carsons, S.E. 2000. Semin. Arthritis Rheum. 29, 252.
      Yamada, K.M. 1989. In Fibronectin, Mosher, D.F., ed., p. 47.
      Rouslahti, E. and Pierschbacher, M.D. 1986. Cell 44, 517.
      Pytela, R., et al. 1985. Cell 40, 191.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-August-2007 RFH
      ApplicationFrozen Sections (0.5-1 µg/ml)
      Immunoblotting (0.5-1 µg/ml)
      Immunofluorescence (see comments)
      Immunoprecipitation (2 µg/mg protein lysate)
      Paraffin Sections (0.5-1 µg/ml, heat pre-treatment required)
      DescriptionProtein G purified mouse monoclonal antibody. Recognizes the ~440 kDa disulfide-linked, dimeric fibronectin.
      BackgroundFibronectin is a dimeric, disulfide-linked glycoprotein of ~440 kDa that is present in basement membranes, extracellular matrix, and blood. Fibronectin contains multiple sites for various ligands and cells including binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. This glycoprotein is involved in cell adhesion, tissue organization, and wound healing. The region of fibronectin that mediates cell adhesion in a majority of cell types is in the C-terminal domain and involves the binding of the Arg-Gly-Asp (RGD) sequence to a specific polypeptide cell surface receptor. Although the mechanisms of fibronectin extracellular matrix assembly are not well understood, specific antibodies have been used to determine the domains involved in matrix assembly. Cellular fibronectin is widely distributed in the stroma of many malignant tumors.
      HostMouse
      Immunogen speciesHuman
      Immunogenrecombinant human fibronectin
      CloneFBN11
      IsotypeIgG₁
      Specieshuman, mouse, rat
      Positive controlSW 156 cells or normal kidney tissue
      Negative controlNormal mouse serum
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsExhibits staining of the connective tissue matrix when used in immunohistochemistry. This antibody is suitable for both reduced and non-reduced protein by immunoblotting. For staining of formalin-fixed tissues, boil in 10 mM citrate buffer, pH 6.0, for 10-20 min, followed by cooling at room temperature for 20 min. This antibody has also been reported to work for immunofluorescence. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesClark, R.A. 2001. Ann. N.Y. Acad. Sci. 936, 355.
      Armstrong, P.B. and Armstrong, M.T. 2000. Biochim. Biophys. Acta 1470, O9.
      Barilla, M.L. and Carsons, S.E. 2000. Semin. Arthritis Rheum. 29, 252.
      Yamada, K.M. 1989. In Fibronectin, Mosher, D.F., ed., p. 47.
      Rouslahti, E. and Pierschbacher, M.D. 1986. Cell 44, 517.
      Pytela, R., et al. 1985. Cell 40, 191.