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CP53 Anti-ICAM-1 (Ab-2) Mouse mAb (W-CAM-1)

This Anti-ICAM-1 (Ab-2) Mouse mAb (W-CAM-1) is validated for use in Cell Inhibition Assays, FC, Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections for the detection of ICAM-1 (Ab-2).

Anti-ICAM-1 (Ab-2) Mouse mAb (W-CAM-1) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Inter-Cellular Adhesion Molecule, Anti-CD54

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CP53
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

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CP53-100UG
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      		 Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~85-115 kDa ICAM-1 protein in endothelial cells and tonsil tissue.
      Catalogue NumberCP53
      Brand Family Calbiochem®
      SynonymsAnti-Inter-Cellular Adhesion Molecule, Anti-CD54
      References
      ReferencesTakami, S., et al. 1998. Circ. 97, 721.
      Craig, A.G., et al. 1997. Infect. Immun. 65, 4580.
      DeGraba, T.J. 1997. Neurology 49, S15.
      Elangbam, C.S., et al. 1997. Vet. Pathol. 34, 61.
      McCormick, C.J., et al. 1997. J. Clin. Invest. 15, 2521.
      Acevedo, A., et al. 1993. Am. J. Pathol. 143, 774.
      De Fougerolles, A.R., et al. 1993. J. Exp. Med. 177, 1187.
      Fawcett, J., et al. 1992. Nature 360, 481.
      Vazeux, R. et al. 1992. Nature 360, 485.
      Boyd, A.W., et al. 1989. Blood 73, 1896.
      Product Information
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
      Positive controlEndothelial cells or tonsil tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesOriginal Clone Boyd, A.W., et al. 1988. PNAS 85, 3095.
      Key Applications Cell Inhibition Assays
      Flow Cytometry
      Frozen Sections
      Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Not Paraffin Sections
      Application NotesCell Inhibition Assays (see comments)
      Flow Cytometry (see comments)
      Frozen Sections (1:50-1:100)
      Immunoblotting (1:200-1:400)
      Immunofluorescence (see comments)
      Immunoprecipitation (10 µl/mg protein lysate)
      Paraffin Sections (not recommended)
      Application CommentsThis antibody will block aggregation of cell lines mediated by ICAM-1 and blocks homotypic binding of purified populations of activated T- and B-lymphocytes and aggregation of mixed T- and B-cell blasts. Will inhibit T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T cell mediated B-cell activation) is significantly inhibited. This antibody will also block elements of both effector arms of the immune system (cytotoxic cell function and Ig production). This antibody has been reported to work for immunofluorescence and flow cytometry studies. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      ImmunogenRaji cells
      ImmunogenHuman
      CloneW-CAM-1
      HostMouse
      IsotypeIgG2b
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CP53-100UG 04055977221008

      Documentation

      Anti-ICAM-1 (Ab-2) Mouse mAb (W-CAM-1) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-ICAM-1 (Ab-2) Mouse mAb (W-CAM-1) Certificates of Analysis

      TitleLot Number
      CP53

      References

      Reference overview
      Takami, S., et al. 1998. Circ. 97, 721.
      Craig, A.G., et al. 1997. Infect. Immun. 65, 4580.
      DeGraba, T.J. 1997. Neurology 49, S15.
      Elangbam, C.S., et al. 1997. Vet. Pathol. 34, 61.
      McCormick, C.J., et al. 1997. J. Clin. Invest. 15, 2521.
      Acevedo, A., et al. 1993. Am. J. Pathol. 143, 774.
      De Fougerolles, A.R., et al. 1993. J. Exp. Med. 177, 1187.
      Fawcett, J., et al. 1992. Nature 360, 481.
      Vazeux, R. et al. 1992. Nature 360, 485.
      Boyd, A.W., et al. 1989. Blood 73, 1896.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision22-August-2007 RFH
      SynonymsAnti-Inter-Cellular Adhesion Molecule, Anti-CD54
      ApplicationCell Inhibition Assays (see comments)
      Flow Cytometry (see comments)
      Frozen Sections (1:50-1:100)
      Immunoblotting (1:200-1:400)
      Immunofluorescence (see comments)
      Immunoprecipitation (10 µl/mg protein lysate)
      Paraffin Sections (not recommended)
      DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing Balb/c splenocytes with p3-NS-1/Ag4-1 (NS1) mouse myeloma cells (see application references). Recognizes the ~85-115 kDa ICAM-1 protein.
      BackgroundCell adhesion molecules are membrane glycoproteins that play a major role in inflammation and neoplastic disease. Four primary groups of adhesion molecules have been described, which includes the integrin receptor family, the immunoglobulin superfamily, selectins, and cadherins. The intercellular adhesion molecules (ICAM-1, ICAM-2, and ICAM-3) belong to the immunoglobulin superfamily that are the counter receptors for the leukocyte integrin, lymphocyte function associated antigen 1 (LFA-1, CD11a/CD18). ICAM-1 (CD54) is a single chain glycoprotein with 7 potential N-linked glycosylation sites. It is expressed in an inducible dependent manner by endothelial and epithelial cells as well as fibroblasts, macrophages, lymphocytes, and leukocytes. ICAM-1 can mediate adhesion by binding to CD11b/CD18 (Mac-1) enhancing antigen specific T-cell activation. ICAM-1 also binds to CD43 and to Plasmodium falciparum infected erythrocytes distinct from the LFA-1 binding site. Studies indicate that ICAM-1 may also be involved in the development of atherosclerotic plaques; increased expression of ICAM-1 has been observed in all types of atherosclerotic lesions except fibrous placques. It is thought that endothelial cell expression of ICAM-1 mediates the recruitment of leukocytes to the lesion as it is suggested by its role in inflammation.
      HostMouse
      Immunogen speciesHuman
      ImmunogenRaji cells
      CloneW-CAM-1
      IsotypeIgG2b
      Specieshuman
      Positive controlEndothelial cells or tonsil tissue
      FormLiquid
      FormulationIn 10 mM PBS, 0.2% BSA, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThis antibody will block aggregation of cell lines mediated by ICAM-1 and blocks homotypic binding of purified populations of activated T- and B-lymphocytes and aggregation of mixed T- and B-cell blasts. Will inhibit T-cell adhesion to normal human endothelial cells. Activation induced by cell-cell contact (mixed lymphocyte reaction, T cell mediated B-cell activation) is significantly inhibited. This antibody will also block elements of both effector arms of the immune system (cytotoxic cell function and Ig production). This antibody has been reported to work for immunofluorescence and flow cytometry studies. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial use, aliquot and freeze (-20°C) for long-term storage if desired. Avoid freeze/thaw.
      Toxicity Standard Handling
      ReferencesTakami, S., et al. 1998. Circ. 97, 721.
      Craig, A.G., et al. 1997. Infect. Immun. 65, 4580.
      DeGraba, T.J. 1997. Neurology 49, S15.
      Elangbam, C.S., et al. 1997. Vet. Pathol. 34, 61.
      McCormick, C.J., et al. 1997. J. Clin. Invest. 15, 2521.
      Acevedo, A., et al. 1993. Am. J. Pathol. 143, 774.
      De Fougerolles, A.R., et al. 1993. J. Exp. Med. 177, 1187.
      Fawcett, J., et al. 1992. Nature 360, 481.
      Vazeux, R. et al. 1992. Nature 360, 485.
      Boyd, A.W., et al. 1989. Blood 73, 1896.
      Application referencesOriginal Clone Boyd, A.W., et al. 1988. PNAS 85, 3095.