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CP35 Anti-PSD-95 (Ab-2) Mouse mAb (7E3-1B8)

This Anti-PSD-95 (Ab-2) Mouse mAb (7E3-1B8) is validated for use in Immunoblotting, Immunofluorescence, Immunoprecipitation for the detection of PSD-95 (Ab-2).

Anti-PSD-95 (Ab-2) Mouse mAb (7E3-1B8) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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CP35
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
RMMonoclonal Antibody

Pricing & Availability

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CP35-100UL
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      Description
      OverviewRecognizes the ~95 kDa PSD95 protein.
      Catalogue NumberCP35
      Brand Family Calbiochem®
      Application Data
      Detection of rat PSD-95 by immunofluorescence. Sample: Rat hippocampal cells. Primary antibody: Anti-PSD-95 (Ab-2) Mouse mAb (7E3-1B8) (Cat. No. CP35) (1:200). Detection: fluorescence.
      References
      ReferencesBrakeman, P.R., et al. 1997. Nature 386, 284.
      Hualing, D., et al. 1997. Nature 386, 279.
      Kennedy, M.B. 1997. Trends in Neurosci. 20, 264.
      Sanes, J. 1997. Curr. Opin. Neurobiol. 7, 93.
      Cho., K.-O., et al. 1992. Neuron 9, 929.
      Product Information
      FormLiquid
      FormulationIn PBS.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesOriginal Clone Kornau, H.-C., et al. 1995. Science 269, 1737.
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Application NotesImmunoblotting (1:2000)
      Immunofluorescence (1:200, indirect)
      Immunoprecipitation (see comments)
      Application CommentsImmunofluorescent staining of rat hippocampal cells shows a staining pattern coincident with NMDA receptor staining at synaptic sites consistent with PSD-95's proposed role in receptor clustering. This antibody has also been reported to work for immunoprecipitation. Fixation with cold methanol is recommended for immunofluorescence. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenrecombinant, rat PSD95
      ImmunogenRat
      Clone7E3-1B8
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Rat
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CP35-100UL 04055977220988

      Documentation

      Anti-PSD-95 (Ab-2) Mouse mAb (7E3-1B8) SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-PSD-95 (Ab-2) Mouse mAb (7E3-1B8) Certificates of Analysis

      TitleLot Number
      CP35

      References

      Reference overview
      Brakeman, P.R., et al. 1997. Nature 386, 284.
      Hualing, D., et al. 1997. Nature 386, 279.
      Kennedy, M.B. 1997. Trends in Neurosci. 20, 264.
      Sanes, J. 1997. Curr. Opin. Neurobiol. 7, 93.
      Cho., K.-O., et al. 1992. Neuron 9, 929.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision21-August-2007 RFH
      ApplicationImmunoblotting (1:2000)
      Immunofluorescence (1:200, indirect)
      Immunoprecipitation (see comments)
      Application Data
      Detection of rat PSD-95 by immunofluorescence. Sample: Rat hippocampal cells. Primary antibody: Anti-PSD-95 (Ab-2) Mouse mAb (7E3-1B8) (Cat. No. CP35) (1:200). Detection: fluorescence.
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with HL-1 mouse myeloma cells (see application references). Recognizes the ~95 kDa PSD-95 protein.
      BackgroundEffective synaptic transmission requires the precise distribution and spatial localization of neurotransmitter receptors and ion channels on the postsynaptic nerve terminal. An understanding of synaptic development is derived largely from studies of the vertebrate skeletal neuromuscular junction. At glutamatergic synapses, the membrane associated cytoskeletal protein PSD-95 (Post Synaptic Density 95kDa) is believed to promote receptor clustering via interactions with the NR2 subunit of the NMDA receptor and the Shaker-type potassium channel. These interactions are likely mediated by multiple repeating PDZ domains (90 amino acid repeats). Two additional proteins, homer and GRIP, have recently been shown to interact with the metabotropic and AMPA receptors, respectively. It is likely that these proteins serve major functions in the molecular scaffolding and signal transduction process, which occurs at the synaptic junction.
      HostMouse
      Immunogen speciesRat
      Immunogenrecombinant, rat PSD95
      Clone7E3-1B8
      IsotypeIgG2a
      Speciesrat
      FormLiquid
      FormulationIn PBS.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsImmunofluorescent staining of rat hippocampal cells shows a staining pattern coincident with NMDA receptor staining at synaptic sites consistent with PSD-95's proposed role in receptor clustering. This antibody has also been reported to work for immunoprecipitation. Fixation with cold methanol is recommended for immunofluorescence. Antibody should be titrated for optimal results in individual systems.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesBrakeman, P.R., et al. 1997. Nature 386, 284.
      Hualing, D., et al. 1997. Nature 386, 279.
      Kennedy, M.B. 1997. Trends in Neurosci. 20, 264.
      Sanes, J. 1997. Curr. Opin. Neurobiol. 7, 93.
      Cho., K.-O., et al. 1992. Neuron 9, 929.
      Application referencesOriginal Clone Kornau, H.-C., et al. 1995. Science 269, 1737.