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CB1044
Sigma-AldrichAnti-OCLN Mouse mAb (1G7)
This Anti-OCLN Mouse mAb (1G7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of OCLN.
More>>This Anti-OCLN Mouse mAb (1G7) is validated for use in ELISA, Immunoblotting, Immunocytochemistry for the detection of OCLN. Less<<
Anti-OCLN Mouse mAb (1G7) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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Description
Overview
Recognizes the ~55-60 kDa OCLN protein in HL-60 and HeLa cells.
Catalogue Number
CB1044
Brand Family
Calbiochem®
Synonyms
Anti-Occludin
Application Data
Detection of human OCLN by immunoblotting. Sample: Whole cell lysate from HL-60 cells. Primary antibody: Anti-OCLN Mouse mAb (1G7) (Cat. No. CB1044) (1-5 µg/ml). Detection: chemiluminescence. Detection of human OCLN by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-OCLN Mouse mAb (1G7) (Cat. No. CB1044) (10 µg/ml). Detection: fluorescence.
ELISA (see comments) Immunoblotting (1-5 µg/ml) Immunocytochemistry (10 µg/ml)
Application Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
A recombinant polypeptide corresponding to amino acids of 423-523 human OCLN, expressed as a GST fusion protein
Clone
1G7
Host
Mouse
Isotype
IgG₁
Species Reactivity
Human
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Blue Ice Only
Toxicity
Regulatory Review
Storage
-20°C
Avoid freeze/thaw
Avoid freeze/thaw
Do not freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
01-November-2010 RFH
Synonyms
Anti-Occludin
Application
ELISA (see comments) Immunoblotting (1-5 µg/ml) Immunocytochemistry (10 µg/ml)
Application Data
Detection of human OCLN by immunoblotting. Sample: Whole cell lysate from HL-60 cells. Primary antibody: Anti-OCLN Mouse mAb (1G7) (Cat. No. CB1044) (1-5 µg/ml). Detection: chemiluminescence. Detection of human OCLN by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-OCLN Mouse mAb (1G7) (Cat. No. CB1044) (10 µg/ml). Detection: fluorescence.
Description
Purified mouse monoclonal antibody. Recognizes the ~55-60 kDa OCLN protein.
Background
OCLN is an integral membrane protein which is located at tight junctions. This protein may be involved in the formation and maintenance of the tight junction.
Host
Mouse
Immunogen
A recombinant polypeptide corresponding to amino acids of 423-523 human OCLN, expressed as a GST fusion protein
Clone
1G7
Isotype
IgG₁
Species
human
Positive control
HL-60 cells, HeLa cells
Form
Liquid
Formulation
In PBS, pH 7.2.
Preservative
None
Comments
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Storage
Avoid freeze/thaw -20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following initial thaw, aliquot and freeze (-20°C).
