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CB1025 Anti-ADD1 Mouse mAb (2C9)

This Anti-ADD1 Mouse mAb (2C9) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of ADD1.

Anti-ADD1 Mouse mAb (2C9) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Aducin1α

CB1025
  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityHostAntibody Type
      HMMonoclonal Antibody
      Description
      OverviewRecognizes the ~75 kDa ADD1 protein in IMR-32 cells and human tonsil tissue.
      Catalogue NumberCB1025
      Brand Family Calbiochem®
      SynonymsAnti-Aducin1α
      Application Data
      Detection of human ADD1 by immunoblotting. Sample: Whole cell lysate from IMR-32 cells. Primary antibody: Anti-ADD1 Mouse mAb (2C9) (Cat. No. CB1025) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human ADD1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human tonsil tissue. Primary antibody: Anti-ADD1 Mouse mAb (2C9) (Cat. No. CB1025) (3 µg/ml). Detection: colorimetric (DAB).
      References
      Product Information
      FormLiquid
      FormulationIn PBS, pH 7.2.
      Positive controlIMR-32 cells, Human tonsil tissue
      PreservativeNone
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Paraffin Sections
      Application NotesELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      ImmunogenFull-length, recombinant human ADD1 (aa 1-663), expressed as a GST fusion protein
      Clone2C9
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Regulatory Review
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      CB1025 0

      Documentation

      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision29-October-2010 RFH
      SynonymsAnti-Aducin1α
      ApplicationELISA (see comments)
      Immunoblotting (1-5 µg/ml)
      Paraffin Sections (3 µg/ml)
      Application Data
      Detection of human ADD1 by immunoblotting. Sample: Whole cell lysate from IMR-32 cells. Primary antibody: Anti-ADD1 Mouse mAb (2C9) (Cat. No. CB1025) (1-5 µg/ml). Detection: chemiluminescence.

      Detection of human ADD1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human tonsil tissue. Primary antibody: Anti-ADD1 Mouse mAb (2C9) (Cat. No. CB1025) (3 µg/ml). Detection: colorimetric (DAB).
      DescriptionPurified mouse monoclonal antibody. Recognizes the ~75 kDa ADD1 protein.
      BackgroundAdducins are a family of cytoskeleton proteins encoded by three genes (α, β, γ). Adducin is a heterodimeric protein that consists of related subunits, which are produced from distinct genes but share a similar structure. Alpha- and beta-adducin include a protease-resistant N-terminal region and a protease-sensitive, hydrophilic C-terminal region. Alpha- and gamma-adducins are ubiquitously expressed. In contrast, beta-adducin is expressed at high levels in brain and hematopoietic tissues. Adducin binds with high affinity to Ca2+/calmodulin and is a substrate for protein kinases A and C.
      HostMouse
      ImmunogenFull-length, recombinant human ADD1 (aa 1-663), expressed as a GST fusion protein
      Clone2C9
      IsotypeIgG₁
      Specieshuman
      Positive controlIMR-32 cells, Human tonsil tissue
      FormLiquid
      FormulationIn PBS, pH 7.2.
      PreservativeNone
      CommentsFor ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Regulatory Review