Survivin is upregulated during liver regeneration in rats and humans and is associated with hepatocyte proliferation.
Hideo A Baba,Jeremias Wohlschlaeger,Klaus J Schmitz,Silvio Nadalin,Hauke Lang,Alexandra Benesch,Yanli Gu,Ali-R Biglarnia,Georgios C Sotiropoulos,Atsushi Takeda,Nobuakira Takeda,Karen von Wnuck Lipinski,Bodo Levkau
Liver international : official journal of the International Association for the Study of the Liver
29
2009
Show Abstract
Survivin regulates cell division and inhibits apoptosis. Liver regeneration is a complex process involving both proliferation and apoptosis. The role of survivin is not well elucidated and no data exist in humans. | 19018973
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Comparison of activated caspase detection methods in the gentamicin-treated chick cochlea.
Christina L Kaiser, Brittany J Chapman, Jessica L Guidi, Caitlin E Terry, Dominic A Mangiardi, Douglas A Cotanche
Hearing research
240
1-11
2008
Show Abstract
Aminoglycoside antibiotics induce caspase-dependent apoptotic death in cochlear hair cells. Apoptosis, a regulated form of cell death, can be induced by many stressors, which activate signaling pathways that result in the controlled dismantling of the affected cell. The caspase family of proteases is activated in the apoptotic signaling pathway and is responsible for cellular destruction. The initiator caspase-9 and the effector caspase-3 are both activated in chick cochlear hair cells following aminoglycoside exposure. We have analyzed caspase activation in the avian cochlea during gentamicin-induced hair cell death to compare two different methods of caspase detection: caspase antibodies and CaspaTag kits. Caspase antibodies bind to the cleaved activated form of caspase-9 or caspase-3 in specific locations in fixed tissue. CaspaTag is a fluorescent inhibitor that binds to a reactive cysteine residue on the large subunit of the caspase heterodimer in unfixed tissue. To induce cochlear hair cell loss, 1-2 week-old chickens received a single injection of gentamicin (300 mg/kg). Chicks were sacrificed 24, 30, 42, 48, 72, or 96 h after injection. Cochleae were dissected and labeled for activated caspase-9 or caspase-3 using either caspase-directed antibodies or CaspaTag kits. Ears were co-labeled with either phalloidin or myosin VI to visualize hair cells and to determine the progression of cochlear damage. The timing of caspase activation was similar for both assays; however, caspase-9 and caspase-3 antibodies labeled only those cells currently undergoing apoptotic cell death. Conversely, CaspaTag-labeled all the cells that have undergone apoptotic cell death and ejection from the sensory epithelium, in addition to those that are currently in the cell death process. This makes CaspaTag ideal for showing an overall pattern or level of cell death over a period of time, while caspase antibodies provide a snapshot of cell death at a specific time point. Full Text Article | 18487027
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