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ABS1591 Anti-SR-alpha Antibody

This Anti-SR-alpha Antibody is validated for use in Western Blotting, Immunoprecipitation for the detection of SR-alpha.

Anti-SR-alpha Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Vesicular Trafficking Gene Symbol: SRPR UniProt Number: P08240
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ABS1591
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, CaWB, IPRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABS1591
      DescriptionAnti-SR-alpha Antibody
      Alternate Names
      • Signal recognition particle receptor subunit alpha
      • Docking protein alpha
      • DP-alpha
      • SR-alpha
      • SRalpha
      Background InformationSignal recognition particle receptor subunit alpha (UniProt P08240; also known as Docking protein alpha, DP-alpha, SR-alpha, SRalpha) is encoded by the SRPR gene (Gene ID 6734) in human. Secretory proteins typically possess N-terminal hydrophobic signal sequences that direct them to the endoplasmic reticulum (ER) membrane, where they are translocated into the ER lumen for subsequent vesicular transport. Signal recognition particle (SRP) delivers target nascent secretory proteins, while they are still being synthesized, together with the associated ribosome to the ER protein translocon (Sec61 complex) via the interaction with SRP receptor (SR) on the ER membrane. SR is a heterodimer composed of a 70 kDa peripheral membrane protein (SR alpha subunit or SRα) and a 30 kDa singlel-trans membrane protein (SR beta subunit or SRβ). SRα contains an N-terminal SRX domain that mediates its association with SRβ and a C-terminal region which has high homology to the NG domain of SRP54, including a functional GTPase domain. SRβ also possesses a GTPase domain, which interacts with SRα SRX domain only when SRα is in the GTP bound state. SRα forms the major interaction interface with SRP.
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal serum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-SR-alpha Antibody is validated for use in Western Blotting, Immunoprecipitation for the detection of SR-alpha.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      Application NotesImmunoprecipitation Analysis: 3 µL from a represenative lot immunoprecipitated SRP receptor alpha subunit (SRα) from canine pancreas tissue rough microsome CHAP extract (Courtesy of Dr. Christopher V. Nicchitta, Duke University Medical Center, Durham, NC).
      Western Blotting Analysis: An 1:5,000 dilution of a representative lot detected SR-alpha in 15 µg HeLa cell lysate (Courtesy of Dr. Christopher V. Nicchitta, Duke University Medical Center, Durham, NC).
      Western Blotting Analysis: A representative lot detected SRP receptor alpha subunit (SRα) in the Brij35-sensitive (BrS), but not in the Brij35-resistant (BrR) fraction of HeLa ER membrfane proteins (Jagannathan, S., et al. (2014). J. Biol. Chem. 2014, 289(37):25907-25924).
      Western Blotting Analysis: A representative lot detected SRP receptor alpha subunit (SRα) in sodium cholate extract (CE) of ribosome-stripped canine pancreas rough microsome (RM) preparation (Migliaccio, G., et al. (1992). J. Cell Biol. 117(1):15-25).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to an internal sequence within the N-terminal half of human SR-alpha.
      EpitopeInternal (N-terminal half).
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityExpected to react with both spliced isoforms of human SR-alpha reported by UniProt (P08240).
      Species Reactivity
      • Human
      • Rat
      • Canine
      Species Reactivity NoteHuman, Rat, Canine. Predicted to react with Mouse and Bovine based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • SRPR
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~75 kDa observed. Target band size appears larger than the calculated molecular weights of 69.81 kDa (isoform 1) and 66.56 kDa (isoform 2).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in canine pancreas tissue rough microsome lysate.

      Western Blotting Analysis: An 1:500 dilution of this antibody detected SR-alpha in canine pancreas tissue rough microsome lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS1591 04055977331264