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ABS1526 Anti-phospho-AKT1 Antibody, Thr479

Anti-phospho-AKT1, Thr479, Cat. No. ABS1526, is a rabbit polyclonal antibody that detects AKT1 phosphorylated on Thr479 and has been tested for use in Western Blotting and Peptide Inhibition Assay.

Anti-phospho-AKT1 Antibody, Thr479 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Gene Symbol: AKT1, PKB, RAC UniProt Number: P31749
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ABS1526
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RPIA, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS1526
      DescriptionAnti-phospho-AKT1 Antibody, Thr479
      Alternate Names
      • AKT alpha
      • Protein kinase B
      • RAC-alpha serine/threonine-protein kinase
      • Protein kinase B alpha
      • PKB
      • Proto-oncogene c-Akt
      • RAC-PK-alpha
      Background InformationRAC-alpha serine/threonine-protein kinase (UniProt: P31749; EC: 2.7.11.1; Protein kinase B, PKB, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT1) is encoded by the AKT1 (also known as PKB, RAC) gene (Gene ID: 207) in human. AKT1 is one of 3 closely related serine/threonine-protein kinases (AKT1, AKT2 and AKT3) that regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. In mammals these 3 isoforms of AKT exhibit a high degree of homology, but differ slightly in the localization of their regulatory phosphorylation sites. AKT1 is constitutively phosphorylated on Ser124, in the region between PH and catalytic domains, and on Thr450, in the C-terminal region in unstimulated cells. Phosphorylation at Thr308 and Ser473 induces complete activation of AKT1. AKT1 contains one PH domain (aa 5-108), one protein kinase domain (aa 150-408), and one AGC-kinase C-terminal domain (aa 409-480). Binding of the PH domain to phosphatidylinositol 3,4,5-trisphosphate (PIP3) results in its targeting to the plasma membrane. AKT1 can be phosphorylated at Ser477/Thr479 by Cdk2/Cyclin A, which locks it into an active conformation. Ser477/Thr479 phosphorylation is believe to trigger Akt1 activation either through enhancing the association between AKT1 and mTORC2 to promote phosphorylation of Ser473, or by functionally compensating for phosphorylated Ser473 to lock AKT1 in its active conformation. AKT1 phosphorylated at Ser477/Thr479 is reported to display increased association with Sin1 and mTOR complexes. (Ref.: Liu, P et al (2014). Nature. 508(7497): 541-545).
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho-AKT1, Thr479, Cat. No. ABS1526, is a rabbit polyclonal antibody that detects AKT1 phosphorylated on Thr479 and has been tested for use in Western Blotting and Peptide Inhibition Assay.
      Key Applications
      • Peptide Inhibition Assay
      • Western Blotting
      Application NotesPeptide Inhibition Analysis: A 1:500 dilution from a representative lot detected phospho-AKT1, Thr479 in RAT2 cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 10 amino acids surrounding Thr479 from the C-terminal region of human AKT1.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects AKT1 phosphorylated on Thr479.
      Species Reactivity
      • Rat
      Species Reactivity NoteRat. Predicted to react with Human and Mouse based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • AKT1
      • PKB
      • RAC
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~56 kDa observed; 55.68 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in RAT2 cells transfected with Src (21-154).

      Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-AKT1, Thr479 in lysate of RAT2 cells transfected with Src (21-154).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABS1526 04054839316050