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ABN1700 Anti-RPGRIP1

Anti-RPGRIP1, Cat. No. ABN1700, is a highly specific rabbit polyclonal antibody that targets X-linked retinitis pigmentosa GTPase regulator-interacting protein 1 and has been tested in Immunofluorescence, Immunocytochemistry, and Western Blotting.

Anti-RPGRIP1 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Rpgrip1 UniProt Number: Q9EPQ2
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ABN1700
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HIF, WB, ICCRbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN1700
      DescriptionAnti-RPGRIP1
      Alternate Names
      • RPGR-interacting protein 1
      • RPGRIP1
      Background InformationX-linked retinitis pigmentosa GTPase regulator-interacting protein 1 (UniProt: Q9EPQ2; also known as RPGR-interacting protein 1; RPGRIP1) is encoded by the Rpgrip1 gene (Gene ID 77945) in mouse species. RPGRIP is expressed in rod and cone photoreceptors and is situated between the axonemal microtubules and the plasma membrane. It consists of an N-terminal region that forms coiled coil structures linked to a C-terminal tail that binds retinitis pigmentosa GTPase regulator (RPGR). RPGRIP1 associates with ciliary axoneme and may function as a scaffolding protein that anchors regulatory complexes, including RPGR. RPGRIP is considered to be essential for RPGR function and is involved in normal disk morphogenesis. Lack of RPGRIP leads to diffusely mislocalized RPGR in photoreceptors and defects in disk formation. Defects in RPGR and RPGRIP are known to cause retinitis pigmentosa and Leber congenital amaurosis (LCA) that leads to retinal dystrophy and loss of vision. Ref: Hong D-H., et al (2001). J. Biol. Chem. 276(15). 12091 12099. Zhao, Y., et al. (2003). Proc. Natl. Acad. Sci. USA 100 (7), 3965-3970.
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in PBS without preservatives with 50% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationAnti-RPGRIP1, Cat. No. ABN1700, is a highly specific rabbit polyclonal antibody that targets X-linked retinitis pigmentosa GTPase regulator-interacting protein 1 and has been tested in Immunofluorescence, Immunocytochemistry, and Western Blotting.
      Key Applications
      • Immunofluorescence
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunofluorescence Analysis: A representative lot detected RPGRIP1 in retinal sections from WT mice (Zhao, Y., et. al. (2003). Proc Natl Acad Sci U S A. 100(7):3965-70).

      Western Blotting Analysis: 1:1,000-1:2,000 of this antibody detected RPGRIP1 in RIPA lysates prepared from mouse retina (Courtesy of Dr. Tiansen at NIH).

      Immunofluorescence Analysis: 1:500-1:2,000 of this antibody detected RPGRIP1 in frozen mouse retina (Courtesy of Dr. Tiansen at NIH).

      Western Blotting Analysis: A representative lot detected RPGRIP1 in WT and mutant (KO) retinal homogenates (Zhao, Y., et. al. (2003). Proc Natl Acad Sci U S A. 100(7):3965-70).

      Immunocytochemistry Analysis: A representative lot detected RPGRIP1 in retina sections (Zhao, Y., et. al. (2003). Proc Natl Acad Sci U S A. 100(7):3965-70).
      Biological Information
      ImmunogenGST-tagged recombinant fragment corresponding to a sequence in the the C-terminal half of mouse RPGRIP.
      EpitopeC-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody detects RPGRIP1 protein in mouse retina and U2OS cells.
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Rpgrip1
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~ 147 kDa observed; 152 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in U2OS cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected RPGRIP1 in 10 µg of U2OS cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.

      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN1700 04054839139222