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ABN1480 Anti-p47-phox Antibody

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ABN1480
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HWB, IHCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABN1480
      DescriptionAnti-p47-phox Antibody
      Alternate Names
      • Neutrophil cytosol factor 1
      • NCF-1
      • 47 kDa neutrophil oxidase factor
      • NCF-47K
      • Neutrophil NADPH oxidase factor 1
      • p47-phox
      • p47-phox
      Background InformationNeutrophil cytosol factor 1 (UniProt Q09014), also known as 47 kDa neutrophil oxidase factor, NADPH oxidase subunit, Ncf-1, NCF-47K, Neutrophil NADPH oxidase factor 1, p47-phox, p47phox) is encoded by the Ncf1 gene in murine species. Activation of NADPH-oxidase dependent superoxide generation requires the participation of several cytosolic factors, including p47-phox, p67-phox, p40-phox and Rac. In the resting cell, p47phox, p67phox, and p40phox form a large molecular weight cytosolic complex. Activation of the NADPH oxidase is initiated by the assembly of p47phox, p67phox, and Rac with cytochrome b558 in a 1:1:1:1 complex in the plasma membrane.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-p47-phox Antibody is an antibody against p47-phox for use in Western Blotting, Immunohistochemistry.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a representative lot detected p47-phox in mouse cerebral cortex and mouse small intestine.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the C-terminus of mouse p47-phox.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteMouse, Human. Predicted to react with Rat based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Ncf1
      Purification MethodAffinity Purfied
      UniProt Number
      Molecular Weight~47 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Raji cell lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected p47-phox in 10 µg of Raji cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN1480 04055977169195