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ABN1380 Anti-Caspr2 Antibody, extracellular domain

Anti-Caspr2 Antibody, extracellular domain, Cat. No. ABN1380, is a highly specific rabbit polyclonal antiserum that targets Caspr2 and has been tested in Immunocytochemistry and Western Blotting.

Anti-Caspr2 Antibody, extracellular domain MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: CNTNAP2, AUTS15, CASPR2, CDFE, KIAA0868 UniProt Number: Q9UHC6
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ABN1380
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWB, ICCRbSerumPolyclonal Antibody
      Description
      Catalogue NumberABN1380
      DescriptionAnti-Caspr2 Antibody, extracellular domain
      Alternate Names
      • Contactin-associated protein-like 2
      • Cell recognition molecule Caspr2
      Background InformationContactin-associated protein-like 2 (UniProt Q9UHC6; also known as Cell recognition molecule Caspr2) is encoded by the CNTNAP2 (also known as AUTS15, CASPR2, CDFE, KIAA0868) gene (Gene ID 26047) in human. Caspr2 is a neurexin superfamily member that functions as a neuronal cell adhesion protein. Originally identified in myelinating nerves, Caspr2 serves to cluster voltage-gated potassium (Kv) channels at the juxtaparanode, a membrane domain adjacent to the node of Ranvier, where it forms a barrier holding nodal components in place. Caspr2 is found in axons, dendrites, dendritic spines and the soma of mature pyramidal neurons in the central nervous system (CNS). Caspr2 and its partner Contactin 2 are found in the synaptic plasma membranes fraction of the forebrain. In the absence of Caspr2, new synaptic spines are unable to stabilize, resulting in decreased spine density and altered spine morphology as well as downregulated AMPA receptor subunit on the spines in mice. CNTNAP2 gene mutations are linked to autism spectrum disorder (ASD) in human. Likewise, Cntnap2-knockout mice exhibit core autism-like deficits including abnormal social behavior and communication, and behavior inflexibility. Caspr2 is produced with a signal peptide (a.a. 1-27), the removal of which yields the mature transmembrane proein with a large extracellular region (a.a. 28-1262), followed by a transmembrane domain (a.a. 1263-1283) and a cytoplasmic tail (human a.a. 1284-1331 and mouse a.a. 1284-1332). The extracellular region contains an N-terminal Discoidin domain (a.k.a. F5/8 type C domain or C2-like domain; human a.a. 35-181), four Laminin G-like domains (human a.a. 216-368, 401-552, 799-963, 1055-1214), two EGF-like domains (human a.a. 554-591 and 963-1002), and a Fibrinogen C-terminal domain (human a.a. 592-798).
      References
      Product Information
      FormatSerum
      PresentationRabbit polyclonal antibody serum with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Caspr2 Antibody, extracellular domain, Cat. No. ABN1380, is a highly specific rabbit polyclonal antiserum that targets Caspr2 and has been tested in Immunocytochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:1000 dilution from a representative lot detected Caspr2 immunoreactivity at the juxtaparanodal region around the nodes of Ranvier by indirect immunofluorescent staining of 0.5% Triton X-100-permeabilized teased fibers prepared from 4% paraformaldehyde-fixed, desheathed wild-type, but not Caspr2-knockout, mouse sciatic nerves (Courtesy of Dr. Elior Peles, Weizmann Institute of Science, Rehovot, Israel).

      Immunocytochemistry Analysis: A representative lot detected Caspr2 immunoreactivity at the juxtaparanodal region around the nodes of Ranvier by indirect immunofluorescent staining of teased fibers prepared from paraformaldehyde-fixed, desheathed wild-type, but not Caspr2-knockout, mouse sciatic nerves (Gordon, A., et al. (2015). Mol. Cell. Sci. In press).

      Western Blotting Analysis: A representative lot detected a lower Caspr2 level in brain tissue lysates from mice with heterozygous Caspr2-knockout than wild-type mice, while no Caspr2 expression was detected in brain samples from mice with homozygous Caspr2-knockout (Gordon, A., et al. (2015). Mol. Cell. Sci. In press).
      Biological Information
      ImmunogenHuman IgG1 Fc-tagged recombinant human Caspr2 extracellular domain fragment.
      Epitopeextracellular domain
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificitySpecificity of this polyclonal antiserum was confirmed by the lack of target detection when using tissue samples from Capr2 knock-out mice. This polyclonal antiserum targets extracellular region absent from human Caspr2 spliced isoform 2 (Uniprot Q9UHC6-2).
      Species Reactivity
      • Mouse
      Species Reactivity NoteMouse. Predicted to react with Human based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • CNTNAP2
      • AUTS15
      • CASPR2
      • CDFE
      • KIAA0868
      Purification MethodUnpurified.
      UniProt Number
      Molecular Weight~180 kDa observed. Target band size appears larger than the calculated molecular weights of 156.3 kDa (human & mouse pro-form) and 154.3/154.2 kDa (human/mouse mature form) due to glycosylation. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse brain tissue lysate.

      Western Blotting Analysis (WB): A 1:5000 dilution of this antibody detected Caspr2 in 10 µg of mouse brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABN1380 04054839034626