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ABE1400 Anti-TFE3

Anti-TFE3, Cat. No. ABE1400, is a highly specific rabbit polyclonal antibody that targets transcription factor E3 (TFE3) and has been tested in Immunocytochemistry and Western Blotting.

Anti-TFE3 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: TFE3, BHLHE33 UniProt Number: P19532
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ABE1400
100 μg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MICC, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE1400
      DescriptionAnti-TFE3
      Alternate Names
      • Transcription factor E3
      • bHLHe33
      • Class E basic helix-loop-helix protein 33
      Background InformationTranscription factor E3 (UniProt P19532; also known as bHLHe33, Class E basic helix-loop-helix protein 33, TFE3) is encoded by the TFE3 (also known as BHLHE33) gene (Gene ID 7030) in human. TFE3 contains adjacent helix-loop-helix (HLH) and leucine zipper (LZ) domains flanked by an upstream basic region. It specifically recognizes and binds E-box sequences (5'-CANNTG-3'). TFE3 is ubiquitously expressed and can directly associate with DNA as either homodimers or heterodimers formed with two related proteins, TFEB or TFEC. In association with TFEB, TFE3 activates the expression of CD40L in T-cells, thereby playing a role in T-cell-dependent antibody responses in activated CD4+ T-cells and in thymus-dependent humoral immunity. TFE3 binds to and activates the microE3 motif of the immunoglobulin heavy-chain enhancer to induce B-cell-specific gene transcription and DNA recombination. TFEB is expressed at low levels in the embryo, but is expressed at high levels in the trophoblast cells of the placenta, where it plays a critical role in regulating normal vascularization. It has been shown that the exit from pluripotency is gated by intracellular redistribution of TFE3. Chromosomal aberrations involving TFE3 have been reported in patients with papillary renal cell carcinoma. Ref.: Betschinger, J., et al. (2013). Cell 153, 335-347.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-TFE3, Cat. No. ABE1400, is a highly specific rabbit polyclonal antibody that targets transcription factor E3 (TFE3) and has been tested in Immunocytochemistry and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution from a representative lot detected TFE3 in 4% paraformaldehyde-fixed, 0.5% Triton X-100-permeablized MEF-1 mouse embryonic fibrolasts.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to a leucine zipper region sequence of human TFE3.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody targets the human TFE3 leucine zipper region sequence.
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TFE3
      • BHLHE33
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~75 kDa observed. Target band size appears larger than the calculated molecular weight of 61.52 kDa due to posttranslational modification. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in NIH/3T3 nuclear extract.

      Western Blotting Analysis: 0.1 µg/mL of this antibody detected TFE3 in 10 µg of NIH/3T3 nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1400 04054839112379