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AB5622-I Anti-MAP-2

Anti-MAP-2, Cat. No. AB5622-I, is a highly specific rabbit polyclonal antibody that targets microtubule-associated protein 2 and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Anti-MAP-2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Gene Symbol: Map2, Mtap2 UniProt Number: P15146
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AB5622-I
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, RICC, WB, IH(P)RbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB5622-I
      DescriptionAnti-MAP-2
      Alternate Names
      • Mirotubule associated protein 2
      Background InformationMicrotubule-associated protein 2 (UniProt: P15146; also known as MAP-2) is encoded by the Map2 (also known as Mtap2) gene (Gene ID: 25595) in Rat. MAPs are a family of proteins that bind to and stabilize microtubules. MAP4 is expressed ubiquitously while MAP1 and MAP2 are expressed primarily in neurons. MAP2 are cytoskeletal proteins that stabilize microtubules and prevents their depolymerization and may also have a stiffening effect on microtubules. MAP-2 plays an important role in brain neuron microtubule assembly. It also associates with neurofilaments and actin filaments and thereby guides interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles. Four different isoforms of MAP2 have been described that bind to microtubules through a domain near the carboxyl terminus. MAP2 isoforms are expressed only in neuronal cells and specifically in the cell bodies and dendrites. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. However, some axons may also have small amounts of MAP-2. MAP-2 usually consists of MAP-2a and MAP-2b, ~280kDa each, and lower molecular weight MAP2c, which is present during neuronal differentiation in the developing nervous system. MAP-2 is a marker for neurons and is uniformly distributed throughout the cell when first expressed in cultured neurons. However, later it is selectively localized as dendritic development proceeds. MAP-2 can be phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2) that results in detachment from microtubules, and their disassembly.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-MAP-2, Cat. No. AB5622-I, is a highly specific rabbit polyclonal antibody that targets microtubule-associated protein 2 and has been tested in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesImmunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected MAP-2 in rat cerebral cortex and rat kidney tissue.

      Western Blotting Analysis: 2 µg/mL from a representative lot detected MAP-2 in 10 µg of rat brain microsomal preparations.

      Immunocytochemistry Analysis: 1 µg/mL from a representative lot detected MAP-2 in rat primary cortex cells.
      Biological Information
      ImmunogenKLH-conjugated three peptides from N-and C-terminal regions of rat MAP-2.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis polyclonal antibody detects MAP2 in Mouse and Rat tissues.
      Species Reactivity
      • Mouse
      • Rat
      Species Reactivity NoteMouse, Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Map2
      • Mtap2
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~280 kDa observed; 202.41 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry in mouse cerebellum tissue.

      Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected MAP-2 in mouse cerebellum tissue.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB5622-I 04054839176487