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AB15332 Anti-Nicotinic Acetylcholine Receptor α7 Antibody

Anti-Nicotinic Acetylcholine Receptor 7, Cat. No. AB15332, is a rabbit polyclonal antibody that detects Nicotinic Acetylcholine Receptor 7 and is tested for use in Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Neurotransmitters & Receptors Gene Symbol: CHRNA7, NACHRA7 UniProt Number: P36544
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AB15332
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, MWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB15332
      ReplacesAB5637
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Nicotinic Acetylcholine Receptor α7 Antibody
      Background InformationNeuronal acetylcholine receptor subunit alpha-7 (UniProt: P36544; also known as -Nicotinic Receptor Alpha 7, Nicotinic AChR alpha7) is encoded by the CHRNA7 (also known as NACHRA7) gene (Gene ID: 1139) in human. Nicotinic acetylcholine receptors are cation-selective, ligand-gated ion channels that mediate fast neurotransmission. They are distributed at the post-synaptic membrane of muscle cells. A similar neuronal-type ACh receptor is also found in the central nervous system and is involved in a wide range of physiological and pathological functions. They are assembled either as homo-pentamers of 7, 8, and 9 or hetero-pentamers of 2 6 in combination with β2 β4 or 9 with 10 subunits. They regulate the flow of sodium, potassium, and calcium ions across the cell membrane. The binding of ligands to these receptors triggers a tertiary conformational transition of neuronal AChRs among functionally distinct resting, open, and desensitized states. Nicotinic AChR 7 is a multi-pass, homopentameric, membrane glycoprotein that is synthesized with a signal peptide (aa 1-22), which is subsequently cleaved off to produce the mature protein that contains an extracellular domain (aa 23-230), four transmembrane domains (aa 231-255; 262-280; 296-317; and 470-490), and one cytoplasmic domain (aa 318-469). (Ref.: Ho, TN., et al. (2020). Front. Neurosci. 14; 609005; Unwin, N. (2013). Q. Rev. Biophys. 46(4); 283-322).
      References
      Product Information
      FormatAffinity Purified
      HS Code3002 15 90
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Nicotinic Acetylcholine Receptor 7, Cat. No. AB15332, is a rabbit polyclonal antibody that detects Nicotinic Acetylcholine Receptor 7 and is tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 28 amino acids from the C-terminal half of human Nicotinic Receptor 7.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody specifically detects Nicotinic Receptor 7. It targets an epitope with 28 amino acids from the C-terminal half.
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. This gene is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from this gene and a novel FAM7A gene.
      Gene Symbol
      • CHRNA7
      • NACHRA7
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P36544 # After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.
      SIZE: 502 amino acids; 56449 Da
      SUBUNIT: Homopentamer. Interacts with RIC3; which is required for proper folding and assembly.
      SUBCELLULAR LOCATION: Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein.
      SIMILARITY: SwissProt: P36544 ## Belongs to the ligand-gated ionic channel (TC 1.A.9) family.
      Molecular WeightTarget molecular weight 52 kDa observed. 56 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Rat brain tissue lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected Nicotinic Acetylcholine Receptor 7 in Rat brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsRecommended storage: +2°C to +8°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB15332 04053252329036

      Documentation

      Anti-Nicotinic Acetylcholine Receptor α7 Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Nicotinic Acetylcholine Receptor α7 Antibody Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-NICOTINIC RECEPTOR ALPHA 7 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2433491 2433491
      RABBIT ANTI-NICOTINIC RECEPTOR ALPHA 7 - 3211114 3211114
      RABBIT ANTI-NICOTINIC RECEPTOR ALPHA 7 - 3933300 3933300
      RABBIT ANTI-NICOTINIC RECEPTOR ALPHA 7 -2630253 2630253
      RABBIT ANTI-NICOTINIC RECEPTOR ALPHA 7 -2723587 2723587
      RABBIT ANTI-NICOTINIC RECEPTOR ALPHA 7 -2762299 2762299
      RABBIT ANTI-NICOTINIC RECEPTOR ALPHA 7 -2794884 2794884
      RABBIT ANTI-NICOTINIC RECEPTOR ALPHA 7 -2884554 2884554
      RABBIT ANTI-NICOTINIC RECEPTOR ALPHA 7 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2040904 2040904
      RABBIT ANTI-NICOTINIC RECEPTOR ALPHA 7 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2066012 2066012

      Data Sheet

      Title
      RABBIT ANTI-NICOTINIC RECEPTOR ALPHA 7