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614850 Topoisomerase I, His•Tag®, Human, Wild Type, Recombinant, S. frugiperda

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Topo I, Wild Type

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614850
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Overview

Replacement Information

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      Description
      OverviewRecombinant, human topoisomerase I fused to a His•Tag® sequence and expressed in S. frugiperda insect cells. It catalyzes the relaxation of both positive and negative supercoiled DNAs without the requirement of energy. In addition to DNA replication and transcriptional activation, DNA topoisomerase I also plays a major role in pre-mRNA splicing, cell cycle and other gene regulatory pathways during cell growth and development. Since Topo I can cause large amounts of single-strand DNA breaks it is an important target for antitumor agents. One unit= 1 ng protein.
      Catalogue Number614850
      Brand Family Calbiochem®
      SynonymsTopo I, Wild Type
      References
      ReferencesPommier, Y., et al. 1998. Biochim. Biophys. Acta 1400, 83.
      Pourquier, P., et al. 1997. J. Biol. Chem. 272, 26441.
      Rossi, F., et al. 1996. Nature 381, 80.
      Sekiguchi, J., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 785.
      Giovanella, B.C., et al. 1989. Science 246, 1046.
      Wang, J,C. 1985. Annu. Rev. Biochem. 54, 665.
      Product Information
      FormLiquid
      Formulation5000 U enzyme in 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 200 µM EDTA, 20% glycerol, pH 7.9 and 1 vial dilution buffer (100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0).
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activity0.1-1 unit (ng) is sufficient for relaxing 0.5 µg of pBR322 supercoiled DNA in a 20 µl reaction.
      Purity≥95% by SDS-PAGE
      Specific Activity1 unit/ng protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw aliquot and freeze (-70°C). For storage of diluted protein, dilute in storage buffer with 500µg/ml molecular biology grade BSA, aliquot, and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      614850-1SET 04055977186345

      Documentation

      Topoisomerase I, His•Tag®, Human, Wild Type, Recombinant, S. frugiperda SDS

      Title

      Safety Data Sheet (SDS) 

      Topoisomerase I, His•Tag®, Human, Wild Type, Recombinant, S. frugiperda Certificates of Analysis

      TitleLot Number
      614850

      References

      Reference overview
      Pommier, Y., et al. 1998. Biochim. Biophys. Acta 1400, 83.
      Pourquier, P., et al. 1997. J. Biol. Chem. 272, 26441.
      Rossi, F., et al. 1996. Nature 381, 80.
      Sekiguchi, J., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 785.
      Giovanella, B.C., et al. 1989. Science 246, 1046.
      Wang, J,C. 1985. Annu. Rev. Biochem. 54, 665.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision15-December-2009 RFH
      SynonymsTopo I, Wild Type
      DescriptionRecombinant, human topoisomerase I fused to a His•Tag® sequence and expressed in S. frugiperda insect cells. It catalyzes the relaxation of both positive and negative supercoiled DNAs without the requirement of energy. In addition to DNA replication and transcriptional activation, DNA topoisomerase I also plays a role in pre-mRNA splicing, cell cycle and other gene regulatory pathways during cell growth and development. DNA Topo I is an important target for ant-tumor agents as a result of the fact that is causes single-strand DNA breaks of the type that are present in most cancers.
      FormLiquid
      Formulation5000 U enzyme in 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 200 µM EDTA, 20% glycerol, pH 7.9 and 1 vial dilution buffer (100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0).
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity≥95% by SDS-PAGE
      Specific activity1 unit/ng protein
      Biological activity0.1-1 unit (ng) is sufficient for relaxing 0.5 µg of pBR322 supercoiled DNA in a 20 µl reaction.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw aliquot and freeze (-70°C). For storage of diluted protein, dilute in storage buffer with 500µg/ml molecular biology grade BSA, aliquot, and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesPommier, Y., et al. 1998. Biochim. Biophys. Acta 1400, 83.
      Pourquier, P., et al. 1997. J. Biol. Chem. 272, 26441.
      Rossi, F., et al. 1996. Nature 381, 80.
      Sekiguchi, J., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 785.
      Giovanella, B.C., et al. 1989. Science 246, 1046.
      Wang, J,C. 1985. Annu. Rev. Biochem. 54, 665.