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539677 PKCθ, His•Tag®, Human, Recombinant, S. frugiperda

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Protein Kinase Cθ

539677
  
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      Overview

      Description
      OverviewFull-length, recombinant, human PKC fused to a His•Tag® sequence and expressed in S. frugiperda insect cells. PKC is a calcium-independent PKC, which is expressed in T lymphocytes and muscle. PKC is reported to be required for T cell activation, proliferation, and cytokine production.
      Catalogue Number539677
      Brand Family Calbiochem®
      SynonymsProtein Kinase Cθ
      References
      ReferencesAltman, A. and Villalba, M. 2002. J. Biochem. 132, 841.
      Villalba, M., et al. 2001. J. Immunol. 166, 5955.
      Baier, G., et al. 1994. Eur. J. Biochem. 225, 195.
      Baier, G., et al. 1993. J. Biol. Chem. 268, 4997.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will transfer 1 nmol phosphate to PKC<sub>ε</sub> Peptide Substrate (Cat. No. 539562) per min at 30°C, pH 7.5
      EC number2.7.1
      FormLiquid
      FormulationIn 150 mM NaCl, 20 mM HEPES, 5 mM DTT, 500 µM EDTA, 500 µM EGTA, 20% glycerol, 0.05% NP-40, pH 7.5.
      Biological Information
      Primary TargetPKCθ
      Purity≥90% by SDS-PAGE
      Specific Activity≥2500 units/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Global Trade Item Number
      Catalogue Number GTIN
      539677 0