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539493 PP1, α-Isoform, Rabbit Muscle, Recombinant, E. coli

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539493
  
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      Overview

      Description
      Overview

      This product has been discontinued.



      Recombinant, rabbit, Mn2+-dependent catalytic subunit of the α-isoform of protein phosphatase 1 expressed in E. coli. Hydrolyzes the phosphate groups on serine and threonine residues and has also been known to hydrolyze phosphates on tyrosine residues. Assists in tobacco mosaic virus-mediated programmed cell death.

      Catalogue Number539493
      Brand Family Calbiochem®
      SynonymsProtein Phosphatase 1, α-Isoform, Phosphorylase Phosphatase
      References
      ReferencesDunigan, D.D., and Madlener, J.C. 1995. Virology 207, 460.
      Zhang, Z., et al. 1993. Mol. Cell. Biochem. 127, 113.
      Zhang, A.J., et al. 1992. J. Biol. Chem. 267, 1484.
      Product Information
      Activity≥500 units/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 nmol of pNPP per minute at 30°C, pH 7.0.
      FormLiquid
      FormulationIn 200 mM NaCl, 50 mM HEPES, 2.5 mM DTT, 1 mM MnCl₂, 0.1 mM EGTA, 50% glycerol, 0.025% TWEEN®-20 Detergent, pH 7.0.
      Biological Information
      Specific Activity13,500 units/mg
      Physicochemical Information
      ContaminantsProtease: none detected
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°c).
      Global Trade Item Number
      Catalogue Number GTIN
      539493 0