Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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Select A Species, Panel Type, Kit or Sample Type
To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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527624
Sigma-AldrichPlasmin, EACA- and Lysine-Free, Human Plasma
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Description
Overview
Native, EACA- and lysine-free plasma from human plasma. Catalyzes the hydrolysis of peptides, arginine and lysine esters and converts fibrin into soluble products.
Catalogue Number
527624
Brand Family
Calbiochem®
References
References
Federici, A.B., et al. 1993. Blood81, 720. Hoffmann, J.J., and Janssen, W.C. 1992. Thromb. Res.67, 711.
Product Information
Unit of Definition
One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of N-tosyl-Gly-Pro-Lys-<i>p</i>NA per min at 25°C, pH 7.8.
EC number
3.4.21.7
Form
Lyophilized
Formulation
Lyophilized from 20 mM sodium phosphate buffer, 10 mg D-mannitol, 10 mg NaCl.
Plasmin, EACA- and Lysine-Free, Human Plasma Certificates of Analysis
Title
Lot Number
527624
References
Reference overview
Federici, A.B., et al. 1993. Blood81, 720. Hoffmann, J.J., and Janssen, W.C. 1992. Thromb. Res.67, 711.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
16-May-2008 RFH
Description
Native, EACA- and lysine-free plasma from human plasma. Catalyzes the hydrolysis of peptides, arginine and lysine esters, and converts fibrin into soluble products.
Form
Lyophilized
Formulation
Lyophilized from 20 mM sodium phosphate buffer, 10 mg D-mannitol, 10 mg NaCl.
Source
Prepared from plasma that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
EC number
3.4.21.7
Specific activity
≥7 units/mg protein
Unit definition
One unit is defined as the amount of enzyme that will hydrolyze 1 µmol of N-tosyl-Gly-Pro-Lys-pNA per min at 25°C, pH 7.8.
Solubility
H₂O
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
From Catalog: Desc. Field- added "Catalyzes the hydrolysis of peptides, arginine and lysine esters and converts fibrin into soluble products.
Toxicity
Standard Handling
Merck USA index
14, 7520
References
Federici, A.B., et al. 1993. Blood81, 720. Hoffmann, J.J., and Janssen, W.C. 1992. Thromb. Res.67, 711.
