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508838 Puromycin, Dihydrochloride, Sterile-Filtered Aqueous Solution, Cell Culture-Tested - CAS 58-58-2 - Calbiochem

An aminonucleoside antibiotic that acts as a prokaryotic and eukaryotic protein synthesis inhibitor.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: (2S)-2-Amino-N-((2S,3S,4R,5R)-5-(6-(dimethylamino)purin-9-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl)-3-(4-methoxyphenyl)

508838
  
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      Overview

      Replacement Information

      Key Spec Table

      CAS #Empirical Formula
      58-58-2C₂₂H₂₉N₇O₅ • 2HCl
      Description
      OverviewAn aminonucleoside antibiotic that acts as a prokaryotic and eukaryotic protein synthesis inhibitor. Resembles the aminoacyl-adenylyl terminus of aminoacyl-tRNA and competes for binding to the A site of the large ribosomal subunit. Its incorporation into a growing polypeptide results in termination of chain elongation and release of the nascent polypeptidyl-puromycin. Useful for selection of mammalian cell lines expressing a transfected pac gene (Streptomyces alboniger), whose product puromycin acetyltransferase, inactivates the drug via acetylation. Induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells. Recommended range for selection of transfected mammalian cells ranges from 1 to 30 µg/ml.
      Catalogue Number508838
      Brand Family Calbiochem®
      Synonyms(2S)-2-Amino-N-((2S,3S,4R,5R)-5-(6-(dimethylamino)purin-9-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl)-3-(4-methoxyphenyl)
      References
      ReferencesChow, S.C., et al. 1995. Exp. Cell Res. 216, 149.
      Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976.
      de la Luna, S. and Ortin, J. 1992. Methods Enzymol. 216, 376.
      Chow, S.C., et al. 1991. Exp. Cell Res. 216, 149.
      Product Information
      CAS number58-58-2
      FormLiquid
      FormulationSupplied at 10 mg/ml in H₂O.
      Hill FormulaC₂₂H₂₉N₇O₅ • 2HCl
      Chemical formulaC₂₂H₂₉N₇O₅ • 2HCl
      ReversibleY
      Quality LevelMQ200
      Applications
      Biological Information
      Primary TargetRNA
      Purity≥98% by HPLC.
      Physicochemical Information
      ContaminantsEndotoxin: ≤10 EU/mg dry weight
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      508838 0

      Documentation

      Puromycin, Dihydrochloride, Sterile-Filtered Aqueous Solution, Cell Culture-Tested - CAS 58-58-2 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Puromycin, Dihydrochloride, Sterile-Filtered Aqueous Solution, Cell Culture-Tested - CAS 58-58-2 - Calbiochem Certificates of Analysis

      TitleLot Number
      508838

      References

      Reference overview
      Chow, S.C., et al. 1995. Exp. Cell Res. 216, 149.
      Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976.
      de la Luna, S. and Ortin, J. 1992. Methods Enzymol. 216, 376.
      Chow, S.C., et al. 1991. Exp. Cell Res. 216, 149.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-May-2014 JSW
      Synonyms(2S)-2-Amino-N-((2S,3S,4R,5R)-5-(6-(dimethylamino)purin-9-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl)-3-(4-methoxyphenyl)
      DescriptionAn aminonucleoside antibiotic that acts as a prokaryotic and eukaryotic protein synthesis inhibitor. Resembles the aminoacyl-adenylyl terminus of aminoacyl-tRNA and competes for binding to the A site of the large ribosomal subunit. Its incorporation into a growing polypeptide results in termination of chain elongation and release of the nascent polypeptidyl-puromycin. Useful for selection of mammalian cell lines expressing a transfected pac gene (Streptomyces alboniger), whose product puromycin acetyltransferase, inactivates the drug via acetylation. Induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells. Recommended range for selection of transfected mammalian cells ranges from 1 to 30 µg/ml.
      FormLiquid
      FormulationSupplied at 10 mg/ml in H₂O.
      Intert gas (Yes/No) Packaged under inert gas
      CAS number58-58-2
      Chemical formulaC₂₂H₂₉N₇O₅ • 2HCl
      Purity≥98% by HPLC.
      ContaminantsEndotoxin: ≤10 EU/mg dry weight
      Storage Protect from light
      Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesChow, S.C., et al. 1995. Exp. Cell Res. 216, 149.
      Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976.
      de la Luna, S. and Ortin, J. 1992. Methods Enzymol. 216, 376.
      Chow, S.C., et al. 1991. Exp. Cell Res. 216, 149.