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481413 Aurora A Kinase, His•Tag®, Human, Recombinant, S. frugiperda

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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481413
  
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      Overview

      Replacement Information
      Description
      Overview

      This product has been discontinued.



      Full-length, recombinant, human Aurora A kinase (amino acids 2-403) (GenBank Accession: NM_003600) fused at the N-terminus to a His•Tag® sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. Aurora A plays an important role in chromosome segregation and cell division.
      Catalogue Number481413
      Brand Family Calbiochem®
      References
      ReferencesOuchi, M., et al. 2004. J. Biol. Chem. 279, 19643.
      Kunitoku, N., et al. 2003. Dev. Cell 5, 853.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substrate per min at 30°C, pH 7.4.
      FormLiquid
      FormulationIn 100 mM NaCl, 25 mM Tris-HCl, 3 mM DTT, 50% glycerol, 0.05% Tween®-20 Detergent, pH 7.5.
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Specific Activity≥120 U/µg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Regulatory Review
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      481413 0

      Documentation

      Aurora A Kinase, His•Tag®, Human, Recombinant, S. frugiperda SDS

      Title

      Safety Data Sheet (SDS) 

      Aurora A Kinase, His•Tag®, Human, Recombinant, S. frugiperda Certificates of Analysis

      TitleLot Number
      481413

      References

      Reference overview
      Ouchi, M., et al. 2004. J. Biol. Chem. 279, 19643.
      Kunitoku, N., et al. 2003. Dev. Cell 5, 853.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision07-September-2007 JSW
      DescriptionFull-length, recombinant, human Aurora A kinase (amino acids 2-403) (GenBank Accession: NM_003600) fused at the N-terminus to a His•Tag® sequence and expressed in S. frugiperda insect cells using a baculovirus expression system. Aurora A plays an important role in chromosome segregation and cell division.
      FormLiquid
      FormulationIn 100 mM NaCl, 25 mM Tris-HCl, 3 mM DTT, 50% glycerol, 0.05% Tween®-20 Detergent, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions
      Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Activity was measured in 50 mM HEPES, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 µM sodium orthovanadate, 500 nM ATP, pH, 7.4 in the presence of 4 µM Aurora A substrate and 0.4 µg/ml Aurora A kinase, 30°C.
      Purity≥90% by SDS-PAGE
      Specific activity≥120 U/µg protein
      Unit definitionOne unit is defined as the amount of enzyme that will phosphorylate 1 pmol of Ser/Thr 1 peptide substrate per min at 30°C, pH 7.4.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Regulatory Review
      ReferencesOuchi, M., et al. 2004. J. Biol. Chem. 279, 19643.
      Kunitoku, N., et al. 2003. Dev. Cell 5, 853.