Millipore Sigma Vibrant Logo

444208 MMP-1, Proenzyme, Human Rheumatoid Synovial Fibroblast

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Matrix Metalloproteinase 1, Human Interstitial Collagenase, Collagenase-1

View Products on Sigmaaldrich.com
444208
View Pricing & Availability

Overview

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
444208-5UG
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      		 Plastic ampoule 5 μg
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      —
      Request Pricing
      		 —
      Add To Favorites
      Description
      OverviewNative proMMP-1 from cultured, human rheumatoid synovial fibroblast. Corresponds to the 56 kDa/52 kDa enzyme. May contain some activated enzyme but requires activation by APMA (Cat. No. 164610) for 30 - 60 min. at 30°C just prior to use to obtain fully activated enzyme (46 kDa/42 kDa). May also undergo autocatalysis to yield a 27 kDa/22 kDa active enzyme. Expressed by a large number of cell types. Cleaves fibrillar type I collagen.
      M.W. 56,000/52,000. Note: 1 mU = 1 milliunit.
      Catalogue Number444208
      Brand Family Calbiochem®
      SynonymsMatrix Metalloproteinase 1, Human Interstitial Collagenase, Collagenase-1
      References
      ReferencesLiepinsh, E., et al. 2003. J. Biol. Chem. 278, 25982.
      Pilcher, B.K., et al. 1997. J. Cell Biol. 137, 1445.
      Vallon, R., et al. 1997. Eur. J. Biochem. 244, 81.
      Marcy, A.I., et al. 1991. Biochemistry 30, 6476.
      Stricklin, et al. 1983. Biochemistry 22, 61.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol 2,4-DNP-Pro-Gln-Gly-Ile-Ala-Gly-Gln-D-Arg-OH per min at 37°C pH 7.0.
      EC number3.4.24.7
      FormLiquid
      FormulationIn 300 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, 1 µM ZnCl₂, 0.05% BRIJ® 35 Detergent, 0.05% NaN₃, pH 7.0.
      Quality LevelMQ100
      Biological Information
      Purity≥90% by SDS-PAGE
      SourcePrepared from culture medium of human rheumatoid synovial fibroblasts that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
      Specific Activity≥15 mU/mg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Contaminantsno other MMP activity detectable
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Global Trade Item Number
      Catalogue Number GTIN
      444208-5UG 04055977205602