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382184 Histone Deacetylase 8, His•Tag®, Human Recombinant, S. frugiperda

Histone Deacetylase 8, His•Tag®, Human Recombinant, S. frugiperda, is a full length HDAC8 fused to His-Tag at the C-terminus. Useful in the study of HDAC8 regulation and for inhibitor screening.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: HDAC8, Human, Recombinant

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382184
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Overview

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382184-50UG
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      		 Plastic ampoule 50 μg
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      Description
      OverviewFull length, recombinant, human histone deacetylase 8 (HDAC8) fused to at the C-terminus to a His•Tag® sequence at and expressed in S. frugiperda insect cells using a baculovirus expression system. HDAC8 phosphorylated in vivo by PKA results in decreased activity, which leads to the hyperacetylation of histones H3 and H4. HDAC8 may play a role in one of the most frequent types of acute myeloid leukemia (AML). The active deacetylase is useful in the study of HDAC8 regulation and for inhibitor screening.
      Catalogue Number382184
      Brand Family Calbiochem®
      SynonymsHDAC8, Human, Recombinant
      References
      ReferencesWaltregny, D., et al. 2005. FASEB J. 19, 966.
      Buggy, J.J., et al. 2000. Biochem J. 350, 199
      Product Information
      Unit of DefinitionOne unit is defined as the the amount of enzyme required to deacetylate 1 picomol fluorescent peptide substrate [Arg-His-Lys(Ac)-Lys(Ac)] per min at 30°C.
      FormLiquid
      FormulationIn 138 mM NaCl, 25 mM Tris-HCl, 10% glycerol, 0.05% TWEEN®-20 detergent, pH 8.0.
      Quality LevelMQ100
      Applications
      ApplicationHistone Deacetylase 8, His•Tag®, Human Recombinant, S. frugiperda, is a full length HDAC8 fused to His-Tag at the C-terminus. Useful in the study of HDAC8 regulation and for inhibitor screening.
      Biological Information
      Purity≥90% by SDS-PAGE
      Specific Activity≥10 units/µg protein
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      382184-50UG 04055977212846

      Documentation

      Histone Deacetylase 8, His•Tag®, Human Recombinant, S. frugiperda SDS

      Title

      Safety Data Sheet (SDS) 

      Histone Deacetylase 8, His•Tag®, Human Recombinant, S. frugiperda Certificates of Analysis

      TitleLot Number
      382184

      References

      Reference overview
      Waltregny, D., et al. 2005. FASEB J. 19, 966.
      Buggy, J.J., et al. 2000. Biochem J. 350, 199
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-May-2008 JSW
      SynonymsHDAC8, Human, Recombinant
      DescriptionFull length, recombinant, human histone deacetylase 8 (HDAC8) fused to at the C-terminus to a His•Tag® sequence at and expressed in S. frugiperda insect cells using a baculovirus expression system. HDAC8 phosphorylated in vivo by PKA results in decreased activity, which leads to the hyperacetylation of histones H3 and H4. HDAC8 may play a role in one of the most frequent types of acute myeloid leukemia (AML). The active deacetylase is useful in the study of HDAC8 regulation and for inhibitor screening.
      FormLiquid
      FormulationIn 138 mM NaCl, 25 mM Tris-HCl, 10% glycerol, 0.05% TWEEN®-20 detergent, pH 8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Recommended reaction conditions
      137 mM NaCl, 25 mM Tris-HCl, 2.7 mM KCl, 1 mM MgCl2, pH 8.0, 100 µM fluorescent substrate [Arg-His-Lys(Ac)-Lys(Ac)], 100 µg/ml BSA, 15 ng/ml HDAC8; incubate at 30°C for 60 min.
      Purity≥90% by SDS-PAGE
      Specific activity≥10 units/µg protein
      Unit definitionOne unit is defined as the the amount of enzyme required to deacetylate 1 picomol fluorescent peptide substrate [Arg-His-Lys(Ac)-Lys(Ac)] per min at 30°C.
      Storage Avoid freeze/thaw
      ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesWaltregny, D., et al. 2005. FASEB J. 19, 966.
      Buggy, J.J., et al. 2000. Biochem J. 350, 199