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354406 Glutaredoxin-S2, E. coli

Glutaredoxin-S2, E. coli, acts as a glutathione-dependent hydrogen donor for ribonucleotide reductase. Useful as a general disulfide reductant for the in vitro study of protein folding mechanisms.

Glutaredoxin-S2, E. coli MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Grx1

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      Overview

      Description
      OverviewNative glutaredoxin-S2 isolated from E. coli. Glutaredoxin functions as a glutathione-dependent hydrogen donor for ribonucleotide reductase. It is useful as a general disulfide reductant for the in vitro study of protein folding mechanisms and has been demonstrated to work in conjunction with protein disulfide isomerase to enhance refolding of scrambled RNase A and RNase T1.
      Catalogue Number354406
      Brand Family Calbiochem®
      SynonymsGrx1
      References
      ReferencesRuoppolo, M., et al. 1997. Biochemistry 36, 12259.
      Sun, C., et al. 1997. Protein Sci. 6, 383.
      Prinz, W.A., et al. 1997. J. Biol. Chem. 272, 15661.
      Holmgren, A. and Alund, F. 1995 Methods Enzymol. 252, 283.
      Hoog, J.O., et al. 1986. Gene 43, 13.
      Product Information
      Unit of DefinitionUnits are defined using a standard HED assay.
      FormLyophilized
      FormulationLyophilized from 0.5% NH₄HCO₃.
      Quality LevelMQ100
      Applications
      ApplicationGlutaredoxin-S2, E. coli, acts as a glutathione-dependent hydrogen donor for ribonucleotide reductase. Useful as a general disulfide reductant for the in vitro study of protein folding mechanisms.
      Biological Information
      Specific Activity≥175 µmole NADPH oxidized/min/mg
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.
      Global Trade Item Number
      Catalogue Number GTIN
      354406 0