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346101 Androgen Receptor, His•Tag®, Human, Recombinant, S. frugiperda

Androgen Receptor, His•Tag®, Human, Recombinant, consists of androgen receptor fused at the N-terminus to a Flag-Tag™ sequence and at the C-terminus to His•Tag® sequence, expressed in S. frugiperda.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: AR, Human, Recombinant, Dihydrotestosterone Receptor, Human, Recombinant

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346101
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346101-5000U
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      Description
      OverviewRecombinant, human androgen receptor (AR) fused at the N-terminus to a Flag-Tag™ sequence and at the C-terminus to His•Tag® sequence, expressed in S. frugiperda insect cells expressed using a baculovirus expression system, and purified by affinity chromatography. The activity of androgen is mediated by the binding of the intracellular androgen receptor which, when activated, translocates to the nucleus and modulates specific gene transcription by interaction with cis-acting hormone response elements in the target genes.
      Catalogue Number346101
      Brand Family Calbiochem®
      SynonymsAR, Human, Recombinant, Dihydrotestosterone Receptor, Human, Recombinant
      References
      ReferencesChen, G., et al. 2005. Prostate 63, 395.
      Estebanez-Perpina, E., et al. 2005, J. Biol. Chem. 280, 8060.
      Gao, S., et al. 2005. Mol. Endocrinol. 19, 1792.
      Hillmer, A.M., et al. 2005. Am. J. Hum. Genet. 77, 140.
      Kinoshita, M., et al. 2005, Int. J. Urol. 12, 390.
      Lim, J.T., et al. 2005. Proc. Natl. Acad. Sci. U.S.A. 102, 5156;
      Marques, R.B., et al. 2005. Int. J. Cancer 117, 221;
      Metzger, E., et al. 2005. Nature 437, 436;
      Murthy, S., et al. 2005. Prostate 64, 362;
      Chang, C., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 5946.
      Product Information
      Activity1.0 unit is equivalent to 1 ng protein. 20 units are sufficient for a gel-mobility shift assay and 100 units are sufficient for a protein-protein interaction assay.
      Unit of DefinitionOne unit is defined as 1 ng purified protein.
      FormLiquid
      FormulationIn 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 200 µM EDTA, 20% glycerol, pH 8.0.
      Quality LevelMQ100
      Applications
      Biological Information
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      346101-5000U 04055977214635

      Documentation

      Androgen Receptor, His•Tag®, Human, Recombinant, S. frugiperda SDS

      Title

      Safety Data Sheet (SDS) 

      Androgen Receptor, His•Tag®, Human, Recombinant, S. frugiperda Certificates of Analysis

      TitleLot Number
      346101

      References

      Reference overview
      Chen, G., et al. 2005. Prostate 63, 395.
      Estebanez-Perpina, E., et al. 2005, J. Biol. Chem. 280, 8060.
      Gao, S., et al. 2005. Mol. Endocrinol. 19, 1792.
      Hillmer, A.M., et al. 2005. Am. J. Hum. Genet. 77, 140.
      Kinoshita, M., et al. 2005, Int. J. Urol. 12, 390.
      Lim, J.T., et al. 2005. Proc. Natl. Acad. Sci. U.S.A. 102, 5156;
      Marques, R.B., et al. 2005. Int. J. Cancer 117, 221;
      Metzger, E., et al. 2005. Nature 437, 436;
      Murthy, S., et al. 2005. Prostate 64, 362;
      Chang, C., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 5946.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision03-November-2008 JSW
      SynonymsAR, Human, Recombinant, Dihydrotestosterone Receptor, Human, Recombinant
      DescriptionRecombinant, human androgen receptor (AR) fused at the N-terminus to a Flag-Tag™ sequence and at the C-terminus to His•Tag® sequence, expressed in S. frugiperda insect cells expressed using a baculovirus expression system, and purified by affinity chromatography. The activity of androgen is mediated by the binding of the intracellular androgen receptor which, when activated, translocates to the nucleus and modulates specific gene transcription by interaction with cis-acting hormone response elements in the target genes.
      FormLiquid
      FormulationIn 100 mM KCl, 20 mM Tris-HCl, 1 mM DTT, 200 µM EDTA, 20% glycerol, pH 8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Activity1.0 unit is equivalent to 1 ng protein. 20 units are sufficient for a gel-mobility shift assay and 100 units are sufficient for a protein-protein interaction assay.
      Unit definitionOne unit is defined as 1 ng purified protein.
      Storage ≤ -70°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesChen, G., et al. 2005. Prostate 63, 395.
      Estebanez-Perpina, E., et al. 2005, J. Biol. Chem. 280, 8060.
      Gao, S., et al. 2005. Mol. Endocrinol. 19, 1792.
      Hillmer, A.M., et al. 2005. Am. J. Hum. Genet. 77, 140.
      Kinoshita, M., et al. 2005, Int. J. Urol. 12, 390.
      Lim, J.T., et al. 2005. Proc. Natl. Acad. Sci. U.S.A. 102, 5156;
      Marques, R.B., et al. 2005. Int. J. Cancer 117, 221;
      Metzger, E., et al. 2005. Nature 437, 436;
      Murthy, S., et al. 2005. Prostate 64, 362;
      Chang, C., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 5946.