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235419 Caspase-3 Cellular Activity Assay Kit

Caspase-3 Cellular Activity Assay Kit MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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235419
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Overview

Key Spec Table

Species ReactivityDetection Methods
A Broad Range Of SpeciesFluorometric or colorimetric

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      Description
      OverviewA convenient assay kit useful for measuring the caspase-3 and caspase-3-like activities in cell extracts using either a colorimetric or fluorometric substrate. Provided in a convenient 96-well format with all reagents necessary for preparing cell extracts, measuring caspase activity and calibrating the assay. In addition, caspase-3 is included for use as a positive control, for testing cell extracts for endogenous inhibitors, or for comparing effects of exogenous inhibitors on cellular activity versus the activity of the purified enzyme.
      Catalogue Number235419
      Brand Family Calbiochem®
      Materials Required but Not Delivered• plate reader capable of measuring A405 to ≥3-decimal accuracy or fluorescent plate reader capable of reading at an excitation of wavelength of 360 nm and an emission wavelength of 460 nm.
      • Pipettor or any multi-channel pipettor capable of pipetting 10-100 µl accurately. Note: dilution of reagents can be made to increase the minimal volume to >10 µl.
      • Ice bucket to keep reagents cold until use.
      • Apoptosis-induced cultured cells.
      • Centrifuge
      • Phosphate buffered saline (PBS).
      References
      ReferencesThornberry, N.A., and Lazebnik, Y. 1998. Science 281, 1312.
      Faleiro, L., et al. 1997. EMBO J. 16, 2271.
      Kothakota, S., et al. 1997. Science 278, 294.
      Nicholson, D.W. 1996. Nat. Biotechnol. 14, 297.
      Schlegel, J., et al. 1996. J. Biol. Chem. 271, 1841.
      Srinivasula, S.M., et al. 1996. Proc. Natl. Acad. Sci. USA 93, 14486.
      Nicholson, D.W., et al. 1995. Nature 376, 37.
      Tewari, M., et al. 1995. Cell 81, 801.
      Fernandes-Alnemi, T., et al. 1994. J.Biol. Chem. 269, 30761.
      Product Information
      Detection methodFluorometric or colorimetric
      Form96 Tests
      Format96-well plate
      Kit containsHuman Recombinant Caspase-3, Cell Lysis Buffer, a DEVD-pNA Colorimetric Substrate, Ac-DEVD-AMC Fluorometric Substrate, Calibration Standard (p-Nitroaniline), AMC Calibration Standard, an Inhibitor, Assay Buffer, one half-volume 96-Well Plate, and a user protocol.
      Quality LevelMQ100
      Biological Information
      Assay time2.5-3 h
      Sample TypeCell extracts
      Species Reactivity
      • A Broad Range Of Species
      Safety Information
      R PhraseR: 23/24/25-63-36/37/38-33

      Toxic by inhalation, in contact with skin and if swallowed.
      Possible risk of harm to the unborn child.
      Irritating to eyes, respiratory system and skin.
      Danger of cumulative effects.
      S PhraseS: 36/37/39-45-26-23

      Wear suitable protective clothing, gloves and eye/face protection.
      In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Do not breathe fumes.
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
      Storage ≤ -70°C
      Storage ConditionsUpon arrival store the 1/2 volume plate at room temperature and the remaining components of the kit at -70°C.
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Supplemental Information
      Kit containsHuman Recombinant Caspase-3, Cell Lysis Buffer, a DEVD-pNA Colorimetric Substrate, Ac-DEVD-AMC Fluorometric Substrate, Calibration Standard (p-Nitroaniline), AMC Calibration Standard, an Inhibitor, Assay Buffer, one half-volume 96-Well Plate, and a user protocol.
      Global Trade Item Number
      Catalogue Number GTIN
      235419-1KIT 07790788048884