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197320 Bcl-2 Converter, D-NuBCP-9-r8, Cell-permeable - Calbiochem

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: (D)-Ac-fsrslhsll-G-NH(CH₂)₅-CO-rrrrrrrr-NH₂, D-NuBCP-9

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197320
  
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      Overview

      Replacement Information

      Key Spec Table

      Empirical Formula
      C₁₀₆H₁₉₁N₄₉O₂₃
      Description
      Overview

      This product has been discontinued.



      An all-D-amino acid version of the Nur77-derived minimum Bcl-2/Bcl-B/Bfl-1-conformation converting sequence is conjugated with a D-Arg octamer to facilitate its cellular delivery. CD binding studies reveal a 1:1 stoichiometric binding and that Bcl-2 unstructured loop region (aa 29-90) as the main D-NuBCP-9 interacting site with possible additional involvement of the BH4 domain. D-NuBCP-9-induced apoptosis is Bcl-2-dependent (100% vs 0% inhibition, respectively, of MEF vs Bcl-2-/- MEF colonies formation by 15 µM D-NuBCP-9) and requires the presence of either Bak or Bax (0% inhibition of Bax-/-Bak-/- MEF colonies formation). D-NuBCP-9-induced apoptosis in HeLa cultures is also demonstrated to be potentiated by 114% and 78%, respectively, upon vector expression of Bcl-B or Bfl-1. When applied to MDA-MB435-xenografted mice on the 10th & 13th day after cancer cell implant, D-NuBCP-9 (620 µg/mouse) reportedly suppressed tumor growth (by 80% on day 55) by inducing cancer cell apoptosis in vivo.
      Catalogue Number197320
      Brand Family Calbiochem®
      Synonyms(D)-Ac-fsrslhsll-G-NH(CH₂)₅-CO-rrrrrrrr-NH₂, D-NuBCP-9
      References
      ReferencesKolluri, S.K., et al. 2008. Cancer Cell 14, 285.
      Product Information
      FormLyophilized solid
      FormulationSupplied as a trifluoroacetate salt.
      Hill FormulaC₁₀₆H₁₉₁N₄₉O₂₃
      Chemical formulaC₁₀₆H₁₉₁N₄₉O₂₃
      Hygroscopic Hygroscopic
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥95% by HPLC
      Physicochemical Information
      Peptide Sequence(D)-Ac-phe-ser-arg-ser-leu-his-ser-leu-leu-Gly-NH-(CH₂)₅-CO-arg-arg-arg-arg-arg-arg-arg-arg-NH₂
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      197320 0

      Documentation

      Bcl-2 Converter, D-NuBCP-9-r8, Cell-permeable - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Bcl-2 Converter, D-NuBCP-9-r8, Cell-permeable - Calbiochem Certificates of Analysis

      TitleLot Number
      197320

      References

      Reference overview
      Kolluri, S.K., et al. 2008. Cancer Cell 14, 285.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision15-September-2009 RFH
      Synonyms(D)-Ac-fsrslhsll-G-NH(CH₂)₅-CO-rrrrrrrr-NH₂, D-NuBCP-9
      DescriptionAn all-D-amino acid version of the Nur77-derived minimum Bcl-2/Bcl-B/Bfl-1-conformation converting sequence is conjugated with a D-Arg octamer to facilitate its cellular delivery. CD binding studies reveal a 1:1 stoichiometric binding and that Bcl-2 unstructured loop region (aa 29-90) as the main D-NuBCP-9 interacting site with possible additional involvement of the BH4 domain. D-NuBCP-9-induced apoptosis is Bcl-2-dependent (100% vs 0% inhibition, respectively, of MEF vs Bcl-2-/- MEF colonies formation by 15 µM D-NuBCP-9) and requires the presence of either Bak or Bax (0% inhibition of Bax-/-Bak-/- MEF colonies formation). D-NuBCP-9-induced apoptosis in HeLa cultures is also demonstrated to be potentiated by 114% and 78%, respectively, upon vector expression of Bcl-B or Bfl-1. When applied to MDA-MB435-xenografted mice on the 10th & 13th day after cancer cell implant, D-NuBCP-9 (620 µg/mouse) reportedly suppressed tumor growth (by 80% on day 55) by inducing cancer cell apoptosis in vivo.
      FormLyophilized solid
      FormulationSupplied as a trifluoroacetate salt.
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₁₀₆H₁₉₁N₄₉O₂₃
      Peptide Sequence(D)-Ac-phe-ser-arg-ser-leu-his-ser-leu-leu-Gly-NH-(CH₂)₅-CO-arg-arg-arg-arg-arg-arg-arg-arg-NH₂
      Purity≥95% by HPLC
      SolubilityH₂O (1 mg/ml) or DMSO (5 mg/ml)
      Storage +2°C to +8°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesKolluri, S.K., et al. 2008. Cancer Cell 14, 285.