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16-263 Anti-Myc Tag Antibody, clone 9E10, Alexa Fluor® 555 conjugate

Detect Myc Tag using this Anti-Myc Tag Antibody, clone 9E10, Alexa Fluor 555 conjugate validated for use in WB & IC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epitope Tags & General Use Research Sub-Category: Epitope Tags Gene Symbol: MYC, C-MYC, c-Myc UniProt Number: P01106
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16-263
100 µg  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      AWB, ICCMAlexaFluor®555Monoclonal Antibody
      Description
      Catalogue Number16-263
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Myc Tag Antibody, clone 9E10, Alexa Fluor® 555 conjugate
      References
      Product Information
      FormatAlexaFluor®555
      Quality LevelMQ100
      Applications
      ApplicationDetect Myc Tag using this Anti-Myc Tag Antibody, clone 9E10, Alexa Fluor 555 conjugate validated for use in WB & IC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Biological Information
      ImmunogenSynthetic peptide corresponding to amino acids 408-437 of the Leucine zipper region of human Myc (AEEQKLISEEDLLRKRREQLKHKLEQLRNS).
      Clone9E10
      HostMouse
      SpecificityMyc and recombinant proteins containing the Myc epitope tag.
      IsotypeIgG1
      Species Reactivity
      • All
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.
      Gene Symbol
      • MYC
      • C-MYC
      • c-Myc
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01106 # Participates in the regulation of gene transcription. Binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
      SIZE: 439 amino acids; 48804 Da
      SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with JARID1A and JARID1B.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Phosphorylated by PRKDC.
      DISEASE: SwissProt: P01106 # Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors. & A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
      SIMILARITY: SwissProt: P01106 ## Contains 1 basic helix-loop-helix (bHLH) domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunocytochemistry on HeLa cells expressing myc-tagged protein.
      Usage Statement
      • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at 4°C from date of shipment. Do not store diluted material. Do not freeze.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      16-263 04053252404146

      Documentation

      Anti-Myc Tag Antibody, clone 9E10, Alexa Fluor® 555 conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Myc Tag Antibody, clone 9E10, Alexa Fluor® 555 conjugate Certificates of Analysis

      TitleLot Number
      Anti-Myc Tag, clone 9E10, Alexa Fluor#174; 555 conjugate (mouse monoclonal IgG1) - 2039758 2039758
      Anti-Myc Tag, clone 9E10, Alexa Fluor#174; 555 conjugate (mouse monoclonal IgG1) - 2262655 2262655
      Anti-Myc Tag, clone 9E10, Alexa Fluor#174; 555 conjugate - DAM1472041 DAM1472041
      Anti-Myc Tag, clone 9E10, Alexa Fluor#174; 555 conjugate - DAM1612159 DAM1612159
      Anti-Myc Tag, clone 9E10, Alexa Fluor#174; 555 conjugate - DAM1739366 DAM1739366
      Anti-Myc Tag, clone 9E10, Alexa Fluor#174; 555 conjugate - JBC1922543 JBC1922543
      Anti-Myc Tag, clone 9E10, Alexa Fluor® 555 conjugate (mouse monoclonal IgG1) - 2393858 2393858
      Anti-Myc Tag, clone 9E10, Alexa Fluor® 555 conjugate (mouse monoclonal IgG1) 3090794
      Anti-Myc Tag, clone 9E10, Alexa Fluor® 555 conjugate (mouse monoclonal IgG1) 2955312
      Anti-Myc Tag, clone 9E10, Alexa Fluor® 555 conjugate (mouse monoclonal IgG1) 3109780
      Show more

      References

      Reference overviewPub Med ID
      Determinants of the HIV-1 core assembly pathway.
      López, CS; Eccles, JD; Still, A; Sloan, RE; Barklis, RL; Tsagli, SM; Barklis, E
      Virology  417  137-46  2011

      Show Abstract       		21676426 21676426

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      Alternative Format

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      Product Families

      Myc Tag Antibodies

      Millipore’s Anti-myc antibodies demonstrate specificity against the myc-tag. See below for related products for myc. All Millipore antibodies are based on the expertise of Upstate & Chemicon.
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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies