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16-225 Anti-Myc Tag Antibody, clone 4A6, Alexa Fluor® 555 conjugate

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16-225
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIF, WB, ICCMAlexaFluor®555Monoclonal Antibody
      Description
      Catalogue Number16-225
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-Myc Tag Antibody, clone 4A6, Alexa Fluor® 555 conjugate
      References
      Product Information
      FormatAlexaFluor®555
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Myc Tag Antibody, clone 4A6, Alexa Fluor 555 conjugate is validated for use in IF, WB, IC for the detection of Myc Tag.
      Key Applications
      • Immunofluorescence
      • Western Blotting
      • Immunocytochemistry
      Biological Information
      ImmunogenKLH-conjugated, synthetic peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc. Clone 4A6
      Cloneclone 4A6
      HostMouse
      SpecificityMyc epitope tag (EQKLISEEDL)
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma. There is evidence to show that alternative translation initiations from an upstream, in-frame non-AUG (CUG) and a downstream AUG start site result in the production of two isoforms with distinct N-termini. The synthesis of non-AUG initiated protein is suppressed in Burkitt's lymphomas, suggesting its importance in the normal function of this gene.
      Gene Symbol
      • MYC
      • C-MYC
      • c-Myc
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01106 # Participates in the regulation of gene transcription. Binds DNA both in a non-specific manner and also specifically to recognizes the core sequence 5'-CAC[GA]TG-3'. Seems to activate the transcription of growth-related genes.
      SIZE: 439 amino acids; 48804 Da
      SUBUNIT: Efficient DNA binding requires dimerization with another bHLH protein. Binds DNA as a heterodimer with MAX. Interacts with TAF1C and SPAG9. Interacts with PARP10. Interacts with JARID1A and JARID1B.
      SUBCELLULAR LOCATION: Nucleus.
      PTM: Phosphorylated by PRKDC.
      DISEASE: SwissProt: P01106 # Overexpression of MYC is implicated in the etiology of a variety of hematopoietic tumors. & A chromosomal aberration involving MYC may be a cause of a form of B-cell chronic lymphocytic leukemia. Translocation t(8;12)(q24;q22) with BTG1.
      SIMILARITY: SwissProt: P01106 ## Contains 1 basic helix-loop-helix (bHLH) domain.
      Molecular Weightvaries
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by immunoblot on RIPA lysates from transfected HeLa cells.
      Usage Statement
      • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditionsstable 1 year at 4°C from date of shipment
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      16-225 04053252477294

      Documentation

      Anti-Myc Tag Antibody, clone 4A6, Alexa Fluor® 555 conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Myc Tag Antibody, clone 4A6, Alexa Fluor® 555 conjugate Certificates of Analysis

      TitleLot Number
      Anti-Myc Tag, clone 4A6, Alexa Fluor#174; 555 conjugate (mouse monoclonal IgG1) - 2109499 2109499
      Anti-Myc Tag, clone 4A6, Alexa Fluor#174; 555 conjugate (mouse monoclonal IgG1) - DAM1416530 DAM1416530
      Anti-Myc Tag, clone 4A6, Alexa Fluor#174; 555 conjugate - DAM1524041 DAM1524041
      Anti-Myc Tag, clone 4A6, Alexa Fluorreg; 555 conjugate - 30439 30439
      Anti-Myc Tag, clone 4A6, Alexa Fluor® 555 conjugate (mouse monoclonal IgG1) 2927611
      Anti-Myc Tag, clone 4A6, Alexa Fluor® 555 conjugate (mouse monoclonal IgG1) 3099048
      Anti-Myc Tag, clone 4A6, Alexa Fluor® 555 conjugate (mouse monoclonal IgG1) - 2227089 2227089
      Anti-Myc Tag, clone 4A6, Alexa Fluor® 555 conjugate - 3207658 3207658
      Anti-Myc Tag, clone 4A6, Alexa Fluor® 555 conjugate - 3499356 3499356
      Anti-Myc Tag, clone 4A6, Alexa Fluor® 555 conjugate - 3589745 3589745

      References

      Reference overviewPub Med ID
      Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems
      Terpe, K
      Appl Microbiol Biotechnol, 60:523-33 (2003)  2003

      12536251 12536251

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      Catalogue Number Description  
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      Product Families

      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies