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09-823 Anti-JMJD1A Antibody

Use Anti-JMJD1A Antibody (Rabbit Polyclonal Antibody) validated in WB, IP to detect JMJD1A also known as lysine (K)-specific demethylase 3A, testis-specific protein A.

Anti-JMJD1A Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histone Modifying Proteins Gene Symbol: JHDM2A, TSGA, JHMD2A, JMJD1, JMJD1A UniProt Number: Q6PCM1
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09-823
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IP, ChIPRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number09-823
      DescriptionAnti-JMJD1A Antibody
      Alternate Names
      • JMJD
      • lysine (K)-specific demethylase 3A
      • testis-specific protein A
      • jumonji domain containing 1A
      • jumonji C domain-containing histone demethylase 2A
      • jumonji domain containing 1
      • Jumonji domain-containing protein 1A
      • JmjC domain-containing histone demethylation protein 2A
      • lysine-specific demethylase 3A
      Background InformationJMJD1A (jumonji domain-containing 1a) is a histone demethylase that plays a key role in the histone code. Functionally, this protein is known to demethylate mono- and dimethylated histoneH3 at lysine 9, while demonstrating virtually no demethylation activity for trimethylated lysine 9 of histone H3. A product of the JHDM2A gene, this protein has been demonstrated to play a role in hypoxia, spermatogenesis, and cell differentiation with loss of function of JMJD1A resulting embryonic stem cell differentiation.
      References
      Product Information
      FormatAffinity Purified
      Control
      • HEK293 nuclear extract.
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationUse Anti-JMJD1A Antibody (Rabbit Polyclonal Antibody) validated in WB, IP to detect JMJD1A also known as lysine (K)-specific demethylase 3A, testis-specific protein A.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesImmunoprecipitation Analysis: 5 µg of a representative lot of this antibody immunoprecipitated JMJD1A in HeLa nuclear extract.

      Western Blot Analysis: A representitive lot of this antibody detected JMJD1A in mouse ESC chromatin extract.
      Biological Information
      ImmunogenGST-tagged recombinant protein corresponding to mouse JMJD1A.
      HostRabbit
      Species Reactivity
      • Human
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummarySummary: This gene encodes a zinc finger protein that contains a jumonji domain and may play a role in hormone-dependent transcriptional activation. Alternative splicing results in multiple transcript variants. [provided by RefSeq].
      Gene Symbol
      • JHDM2A
      • TSGA
      • JHMD2A
      • JMJD1
      • JMJD1A
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Histone demethylase that specifically demethylates 'Lys-9' of histone H3, thereby playing a central role in histone code. Preferentially demethylates mono- and dimethylated H3 'Lys-9' residue, with a preference for dimethylated residue, while it has weak or no activity on trimethylated H3 'Lys-9'. Demethylation of Lys residue generates formaldehyde and succinate. Involved in hormone-dependent transcriptional activation, by participating in recruitment to androgen-receptor target genes, resulting in H3 'Lys-9' demethylation and transcriptional activation. Involved in spermatogenesis by regulating expression of target genes such as PRM1 and TMP1 which are required for packaging and condensation of sperm chromatin. Involved in obesity resistance through regulation of metabolic genes such as PPARA and UCP1.

      COFACTOR: Binds 1 Fe2+ ion per subunit.

      SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Note: Nuclear in round spermatids. When spermatids start to elongate, localizes to the cytoplasm where it forms distinct foci which disappear in mature spermatozoa (By similarity).

      DOMAIN: The JmjC domain and the C6-type zinc-finger are required for the demethylation activity. Leu-Xaa-Xaa-Leu-Leu (LXXLL) motifs are known to mediate the association with nuclear receptors (By similarity).

      SEQUENCE SIMILARITIES: Belongs to the JHDM2 histone demethylase family. Contains 1 JmjC domain.

      SEQUENCE CAUTION: The sequence BAA34462.2 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.
      Molecular Weight~147 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HEK293 nuclear extract.

      Western Blot Analysis: 1 µg/mL of this antibody detected JMJD1A on 10 µg of HEK293 nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      09-823 04053252750199